Advances in Therapy

, Volume 33, Issue 5, pp 824–833 | Cite as

Local Injection of Bone Mesenchymal Stem Cells and Fibrin Glue Promotes the Repair of Bone Atrophic Nonunion In Vivo

  • Chenguang Hao
  • Yanlong Wang
  • Lin Shao
  • Jianyu Liu
  • Lin Chen
  • Zhenyu ZhaoEmail author
Original Research



This study aimed to evaluate the efficacy of local injection of bone mesenchymal stem cells (BMSCs) and fibrin glue in the treatment of atrophic nonunion in an animal model.


Thirty-six male Lewis rats were randomly assigned into three groups: Group A (control group), Group B (atrophic nonunion group), and Group C (experimental group). All the rats underwent femoral osteotomy of the right hind limb, and stabilized with a custom-designed external fixator. Atrophic nonunion of the rats in Group B and C was induced by cauterization of the periosteum and bone marrow removal, and repaired by injection of fibrin glue and BMSCs-seeded fibrin glue, respectively. The surgically treated femurs were assessed by radiographic and histological analysis, and biomechanical test.


During the follow-up period, the external fixator maintained correct placement and all the femurs retained normal positioning. Eight weeks postoperatively, atrophic nonunion was detected in Group B, with the presence of fibrous connective tissue in the osteotomy gap. The femurs in Group C demonstrated complete bony bridging of the osteotomy gap, with the formation of plenty of woven bone.


The repair of bone atrophic nonunion can be promoted through local injection of BMSCs and fibrin glue.


Animal study Atrophic nonunion Bone mesenchymal stem cells External fixator Orthopedics Tissue engineering 



This study was supported by grants from the Education Department of Heilongjiang Province, China (No. 12521269), The Health Department of Heilongjiang Province, China (No. 2012-595) and Harbin Municipal Science and Technology Bureau (No. 2013RFQYJ063). The article processing charges for this publication were funded by the authors. All named authors meet the International Committee of Medical Journal Editors (ICMJE) criteria for authorship for this manuscript, take responsibility for the integrity of the work as a whole, and have given final approval for the version to be published.


Chenguang Hao, Yanlong Wang, Lin Shao, Jianyu Liu, Lin Chen, and Zhenyu Zhao have no conflict of interest to declare.

Compliance with Ethics Guidelines

The present study was approved by the Institutional Animal Care and Use Committee of the Harbin Medical University.


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Copyright information

© Springer Healthcare 2016

Authors and Affiliations

  • Chenguang Hao
    • 1
  • Yanlong Wang
    • 1
  • Lin Shao
    • 1
  • Jianyu Liu
    • 1
  • Lin Chen
    • 1
  • Zhenyu Zhao
    • 1
    Email author
  1. 1.Department of OrthopaedicsThe Second Affiliated Hospital of Harbin Medical UniversityHarbinChina

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