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Centrosome Inheritance Does Not Regulate Cell Fate in Granule Neuron Progenitors of the Developing Cerebellum

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Abstract

An inherent asymmetry exists between the two centrosomes of a dividing cell. One centrosome is structurally more mature (mother centrosome) than the other (daughter centrosome). Post division, one daughter cell inherits the mother centrosome while the other daughter cell inherits the daughter centrosome. Remarkably, the kind of centrosome inherited is associated with cell fate in several developmental contexts such as in radial glial progenitors in the developing mouse cortex, Drosophila neuroblast divisions and in Drosophila male germline stem cells. However, the role of centrosome inheritance in granule neuron progenitors in the developing cerebellum has not been investigated. Here, we show that mother and daughter centrosomes do exist in these progenitors, and the amount of pericentriolar material (PCM) each centrosome possesses is different. However, we failed to observe any correlation between the fate adopted by the daughter cell and the nature of centrosome it inherited.

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Acknowledgements

The authors thank Parthiv Haldipur and Ajit Ray (National Brain Research Centre, India) for their inputs.

Funding

SM received funding from the Indo-French Centre for the Promotion of Advanced Research (CEFIPRA, grant no. 4903-02). KC received financial support from the Department of Biotechnology, Government of India (DBT Neurobiology Task Force, grant no. 0376). NR was supported by SwarnaJayanti Fellowship from the Department of Science and Technology, Government of India, and the DBT-IISc partnership program. AC received financial support from the Council of Scientific and Industrial Research (CSIR), Government of India.

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  1. Shyamala Mani

    Present address: Curadev Pharma, Pvt. Ltd., B-87, Sector 83, Noida, Uttar Pradesh, 201305, India

Authors and Affiliations

  1. Centre for Neuroscience, Indian Institute of Science, Bengaluru, 560012, India

    Anindo Chatterjee, Kaviya Chinnappa, Narendrakumar Ramanan & Shyamala Mani

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  1. Anindo Chatterjee
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  2. Kaviya Chinnappa
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Correspondence to Anindo Chatterjee or Shyamala Mani.

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Chatterjee, A., Chinnappa, K., Ramanan, N. et al. Centrosome Inheritance Does Not Regulate Cell Fate in Granule Neuron Progenitors of the Developing Cerebellum. Cerebellum 17, 685–691 (2018). https://doi.org/10.1007/s12311-018-0935-4

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  • DOI: https://doi.org/10.1007/s12311-018-0935-4

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