Heterotopic Purkinje Cells: a Comparative Postmortem Study of Essential Tremor and Spinocerebellar Ataxias 1, 2, 3, and 6

  • Elan D. Louis
  • Sheng-Han Kuo
  • William J. Tate
  • Geoffrey C. Kelly
  • Jesus Gutierrez
  • Etty P. Cortes
  • Jean-Paul G. Vonsattel
  • Phyllis L. Faust
Original Paper

Abstract

Essential tremor (ET) is among the most common neurological diseases. Postmortem studies have noted a series of pathological changes in the ET cerebellum. Heterotopic Purkinje cells (PCs) are those whose cell body is mis-localized in the molecular layer. In neurodegenerative settings, these are viewed as a marker of the progression of neuronal degeneration. We (1) quantify heterotopias in ET cases vs. controls, (2) compare ET cases to other cerebellar degenerative conditions (spinocerebellar ataxias (SCAs) 1, 2, 3, and 6), (3) compare these SCAs to one another, and (4) assess heterotopia within the context of associated PC loss in each disease. Heterotopic PCs were quantified using a standard LH&E-stained section of the neocerebellum. Counts were normalized to PC layer length (n-heterotopia count). It is also valuable to consider PC counts when assessing heterotopia, as loss of PCs extends both to normally located as well as heterotopic PCs. Therefore, we divided n-heterotopias by PC counts. There were 96 brains (43 ET, 31 SCA [12 SCA1, 7 SCA2, 7 SCA3, 5 SCA6], and 22 controls). The median number of n-heterotopias in ET cases was two times higher than that of the controls (2.6 vs. 1.2, p < 0.05). The median number of n-heterotopias in the various SCAs formed a spectrum, with counts being highest in SCA3 and SCA1. In analyses that factored in PC counts, ET had a median n-heterotopia/Purkinje cell count that was three times higher than the controls (0.35 vs. 0.13, p < 0.01), and SCA1 and SCA2 had counts that were 5.5 and 11 times higher than the controls (respective p < 0.001). The median n-heterotopia/PC count in ET was between that of the controls and the SCAs. Similarly, the median PC count in ET was between that of the controls and the SCAs; the one exception was SCA3, in which the PC population is well known to be preserved. Heterotopia is a disease-associated feature of ET. In comparison, several of the SCAs evidenced even more marked heterotopia, although a spectrum existed across the SCAs. The median n-heterotopia/PC count and median PC in ET was between that of the controls and the SCAs; hence, in this regard, ET could represent an intermediate state or a less advanced state of spinocerebellar atrophy.

Keywords

Essential tremor Spinocerebellar ataxia Cerebellum Neurodegenerative Purkinje cell Pathology Heterotopia 

References

  1. 1.
    Louis ED, Ferreira JJ. How common is the most common adult movement disorder? Update on the worldwide prevalence of essential tremor. Mov Disord. 2010;25:534–41.CrossRefPubMedGoogle Scholar
  2. 2.
    Dogu O, Sevim S, Camdeviren H, Sasmaz T, Bugdayci R, Aral M, et al. Prevalence of essential tremor: door-to-door neurologic exams in Mersin Province. Turkey Neurology. 2003;61:1804–6.CrossRefPubMedGoogle Scholar
  3. 3.
    Benito-Leon J, Bermejo-Pareja F, Morales JM, Vega S, Molina JA. Prevalence of essential tremor in three elderly populations of central Spain. Mov Disord. 2003;18:389–94.CrossRefPubMedGoogle Scholar
  4. 4.
    Louis ED. The primary type of tremor in essential tremor is kinetic rather than postural: cross-sectional observation of tremor phenomenology in 369 cases. Eur J Neurol. 2013;20:725–7.CrossRefPubMedGoogle Scholar
  5. 5.
    Louis ED, Frucht SJ, Rios E. Intention tremor in essential tremor: prevalence and association with disease duration. Mov Disord. 2009;24:626–7.CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Koster B, Deuschl G, Lauk M, Timmer J, Guschlbauer B, Lucking CH. Essential tremor and cerebellar dysfunction: abnormal ballistic movements. J Neurol Neurosurg Psychiatry. 2002;73:400–5.CrossRefPubMedPubMedCentralGoogle Scholar
  7. 7.
    Singer C, Sanchez-Ramos J, Weiner WJ. Gait abnormality in essential tremor. Mov Disord. 1994;9:193–6.CrossRefPubMedGoogle Scholar
  8. 8.
    Hoskovcova M, Ulmanova O, Sprdlik O, Sieger T, Novakova J, Jech R, et al. Disorders of balance and gait in essential tremor are associated with midline tremor and age. Cerebellum. 2012;12:27–34.CrossRefGoogle Scholar
  9. 9.
    Louis ED, Galecki M, Rao AK. Four essential tremor cases with moderately impaired gait: how impaired can gait be in this disease? Tremor Other Hyperkinet Mov (N Y). 2013;3. pii: tre-03-200-4597-1. doi: 10.7916/D8QV3K7G. eCollection 2013.
  10. 10.
    Stolze H, Petersen G, Raethjen J, Wenzelburger R, Deuschl G. The gait disorder of advanced essential tremor. Brain. 2001;124:2278–86.CrossRefPubMedGoogle Scholar
  11. 11.
    Helmchen C, Hagenow A, Miesner J, Sprenger A, Rambold H, Wenzelburger R, et al. Eye movement abnormalities in essential tremor may indicate cerebellar dysfunction. Brain. 2003;126:1319–32.CrossRefPubMedGoogle Scholar
  12. 12.
    Gitchel GT, Wetzel PA, Baron MS. Slowed saccades and increased square wave jerks in essential tremor. Tremor Other Hyperkinet Mov (N Y). 2013;3. pii: tre-03-178-4116-2. doi: 10.7916/D8251GXN. eCollection 2013.
  13. 13.
    Bares M, Lungu OV, Husarova I, Gescheidt T. Predictive motor timing performance dissociates between early diseases of the cerebellum and Parkinson’s disease. Cerebellum. 2010;9:124–35.CrossRefPubMedGoogle Scholar
  14. 14.
    Benito-Leon J, Labiano-Fontcuberta A. Linking essential tremor to the cerebellum: clinical evidence. Cerebellum. 2016;15:253–62.CrossRefPubMedGoogle Scholar
  15. 15.
    Cerasa A, Quattrone A. Linking essential tremor to the cerebellum-neuroimaging evidence. Cerebellum. 2016;15:263–75.CrossRefPubMedGoogle Scholar
  16. 16.
    Passamonti L, Cerasa A, Quattrone A. Neuroimaging of essential tremor: what is the evidence for cerebellar involvement? Tremor Other Hyperkinet Mov (N Y). 2012;2. pii: 02–67–421-3. doi: 10.7916/D8F76B8G. Epub 2012 Sep 17.
  17. 17.
    Louis ED, Shungu DC, Chan S, Mao X, Jurewicz EC, Watner D. Metabolic abnormality in the cerebellum in patients with essential tremor: a proton magnetic resonance spectroscopic imaging study. Neurosci Lett. 2002;333:17–20.CrossRefPubMedGoogle Scholar
  18. 18.
    Sharifi S, Nederveen AJ, Booij J, van Rootselaar AF. Neuroimaging essentials in essential tremor: a systematic review. Neuroimage Clin. 2014;5:217–31.CrossRefPubMedPubMedCentralGoogle Scholar
  19. 19.
    Quattrone A, Cerasa A, Messina D, Nicoletti G, Hagberg GE, Lemieux L, et al. Essential head tremor is associated with cerebellar vermis atrophy: a volumetric and voxel-based morphometry MR imaging study. Am J Neuroradiol. 2008;29:1692–7.CrossRefPubMedGoogle Scholar
  20. 20.
    Louis ED. Linking essential tremor to the cerebellum: neuropathological evidence. Cerebellum. 2016;15:235–42.CrossRefPubMedGoogle Scholar
  21. 21.
    Louis ED, Faust PL, Vonsattel JP, Honig LS, Rajput A, Robinson CA, et al. Neuropathological changes in essential tremor: 33 cases compared with 21 controls. Brain. 2007;130:3297–307.CrossRefPubMedGoogle Scholar
  22. 22.
    Babij R, Lee M, Cortes E, Vonsattel JP, Faust PL, Louis ED. Purkinje cell axonal anatomy: quantifying morphometric changes in essential tremor versus control brains. Brain. 2013;136:3051–61.CrossRefPubMedPubMedCentralGoogle Scholar
  23. 23.
    Erickson-Davis CR, Faust PL, Vonsattel JP, Gupta S, Honig LS, Louis ED. “Hairy baskets” associated with degenerative Purkinje cell changes in essential tremor. J Neuropathol Exp Neurol. 2010;69:262–71.CrossRefPubMedPubMedCentralGoogle Scholar
  24. 24.
    Kuo SH, Tang G, Louis ED, Ma K, Babji R, Balatbat M, et al. Lingo-1 expression is increased in essential tremor cerebellum and is present in the basket cell pinceau. Acta Neuropathol. 2013;125:879–89.CrossRefPubMedPubMedCentralGoogle Scholar
  25. 25.
    Louis ED, Babij R, Lee M, Cortes E, Vonsattel JP. Quantification of cerebellar hemispheric Purkinje cell linear density: 32 ET cases versus 16 controls. Mov Disord. 2013;28:1854–9.CrossRefPubMedGoogle Scholar
  26. 26.
    Choe M, Cortes E, Vonsattel JG, Kuo SH, Faust PL, Louis ED. Purkinje cell loss in essential tremor: random sampling quantification and nearest neighbor analysis. Mov Disord. 2016;31:393–401.CrossRefPubMedPubMedCentralGoogle Scholar
  27. 27.
    Symanski C, Shill HA, Dugger B, Hentz JG, Adler CH, Jacobson SA, et al. Essential tremor is not associated with cerebellar Purkinje cell loss. Mov Disord. 2014;29:496–500.CrossRefPubMedGoogle Scholar
  28. 28.
    Rajput AH, Robinson CA, Rajput ML, Robinson SL, Rajput A. Essential tremor is not dependent upon cerebellar Purkinje cell loss. Parkinsonism Relat Disord. 2012;18:626–8.CrossRefPubMedGoogle Scholar
  29. 29.
    Kuo SH, Erickson-Davis C, Gillman A, Faust PL, Vonsattel JP, Louis ED. Increased number of heterotopic Purkinje cells in essential tremor. J Neurol Neurosurg Psychiatry. 2011;82:1038–40.CrossRefPubMedGoogle Scholar
  30. 30.
    Nakamura R, Kurita K, Kawanami T, Kato T. An immunohistochemical study of Purkinje cells in a case of hereditary cerebellar cortical atrophy. Acta Neuropathol. 1999;97:196–200.CrossRefPubMedGoogle Scholar
  31. 31.
    Gomez CM, Thompson RM, Gammack JT, Perlman SL, Dobyns WB, Truwit CL, et al. Spinocerebellar ataxia type 6: gaze-evoked and vertical nystagmus, Purkinje cell degeneration, and variable age of onset. Ann Neurol. 1997;42:933–50.CrossRefPubMedGoogle Scholar
  32. 32.
    Yamada M, Sato T, Tsuji S, Takahashi H. CAG repeat disorder models and human neuropathology: similarities and differences. Acta Neuropathol. 2008;115:71–86.CrossRefPubMedGoogle Scholar
  33. 33.
    Mangaru Z, Salem E, Sherman M, Van Dine SE, Bhambri A, Brumberg JC, et al. Neuronal migration defect of the developing cerebellar vermis in substrains of C57BL/6 mice: cytoarchitecture and prevalence of molecular layer heterotopia. Dev Neurosci. 2013;35:28–39.CrossRefPubMedGoogle Scholar
  34. 34.
    Bottini AR, Gatti RA, Wirenfeldt M, Vinters HV. Heterotopic Purkinje cells in ataxia-telangiectasia. Neuropathology. 2012;32:23–9.CrossRefPubMedGoogle Scholar
  35. 35.
    Shahbazian MD, Orr HT, Zoghbi HY. Reduction of Purkinje cell pathology in SCA1 transgenic mice by p53 deletion. Neurobiol Dis. 2001;8:974–81.CrossRefPubMedGoogle Scholar
  36. 36.
    Yang Q, Hashizume Y, Yoshida M, Wang Y, Goto Y, Mitsuma N, et al. Morphological Purkinje cell changes in spinocerebellar ataxia type 6. Acta Neuropathol. 2000;100:371–6.CrossRefPubMedGoogle Scholar
  37. 37.
    Goffinet AM, So KF, Yamamoto M, Edwards M, Caviness VS Jr. Architectonic and hodological organization of the cerebellum in reeler mutant mice. Brain Res. 1984;318:263–76.CrossRefPubMedGoogle Scholar
  38. 38.
    Kuo SH, Wang J, Tate WJ, Pan MK, Kelly GC, Gutierrez J, et al. Cerebellar pathology in early onset and late onset essential tremor. Cerebellum. 2017;16:473–82.CrossRefPubMedGoogle Scholar
  39. 39.
    Harasymiw JW, Bean P. Identification of heavy drinkers by using the early detection of alcohol consumption score. Alcohol Clin Exp Res. 2001;25:228–35.CrossRefPubMedGoogle Scholar
  40. 40.
    Louis ED, Babij R, Ma K, Cortes E, Vonsattel JP. Essential tremor followed by progressive supranuclear palsy: postmortem reports of 11 patients. J Neuropathol Exp Neurol. 2013;72:8–17.CrossRefPubMedPubMedCentralGoogle Scholar
  41. 41.
    Stoodley CJ, Schmahmann JD. Functional topography in the human cerebellum: a meta-analysis of neuroimaging studies. NeuroImage. 2009;44:489–501.CrossRefPubMedGoogle Scholar
  42. 42.
    Koeppen AH, Ramirez RL, Bjork ST, Bauer P, Feustel PJ. The reciprocal cerebellar circuitry in human hereditary ataxia. Cerebellum. 2013;12:493–503.CrossRefPubMedPubMedCentralGoogle Scholar
  43. 43.
    Rub U, Schols L, Paulson H, Auburger G, Kermer P, Jen JC, et al. Clinical features, neurogenetics and neuropathology of the polyglutamine spinocerebellar ataxias type 1, 2, 3, 6 and 7. Prog Neurobiol. 2013;104:38–66.CrossRefPubMedGoogle Scholar
  44. 44.
    Lin CY, Louis ED, Faust PL, Koeppen AH, Vonsattel JP, Kuo SH. Abnormal climbing fibre-Purkinje cell synaptic connections in the essential tremor cerebellum. Brain. 2014;137:3149–59.CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  • Elan D. Louis
    • 1
    • 2
    • 3
  • Sheng-Han Kuo
    • 4
  • William J. Tate
    • 5
  • Geoffrey C. Kelly
    • 5
  • Jesus Gutierrez
    • 1
  • Etty P. Cortes
    • 5
    • 6
  • Jean-Paul G. Vonsattel
    • 5
    • 6
  • Phyllis L. Faust
    • 5
  1. 1.Department of Neurology, Yale School of MedicineYale UniversityNew HavenUSA
  2. 2.Department of Chronic Disease Epidemiology, Yale School of Public HealthYale UniversityNew HavenUSA
  3. 3.Center for Neuroepidemiology and Clinical Neurological Research, Yale School of MedicineYale UniversityNew HavenUSA
  4. 4.Department of Neurology, College of Physicians and SurgeonsColumbia UniversityNew YorkUSA
  5. 5.Department of Pathology and Cell BiologyColumbia University Medical Center and the New York Presbyterian HospitalNew YorkUSA
  6. 6.Taub Institute for Research on Alzheimer’s Disease and the Aging BrainColumbia UniversityNew YorkUSA

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