LGI1 is Involved in the Development of Mouse Brain
Mutations in leucine-rich glioma inactivated 1 (LGI1) are linked to human autosomal dominant lateral temporal lobe epilepsy. It has been shown that LGI1 prevents the inactivation of voltage-gated potassium channels, mediates postnatal maturation of glutamatergic synapses, and regulates excitatory neurotransmission. However, other functions of LGI1 in the central nervous system have not been elucidated. We found that LGI1 is involved in the development of the cerebellum and cortex. The thickness of external granule layer was reduced, and foliation was affected in the cerebellum of LGI1 knockout mice. Double staining with Pax6 and BrdU showed a significant inhibition of proliferation of granule cell precursors of knockout embryos. The differentiation of radial glia cells was also suppressed in knockout mice, as shown by increased radial glial cells and decreased Bergmann glias in the areas of the cerebellum and cortex. Thus, our data demonstrate that LGI1 may be an essential player in the development of the brain.
KeywordsLGI1 Cerebellum GFAP Pax6 BLBP
We thank Dr. John Cowell (Georgia Health Sciences University, Augusta, AT) for providing the LGI1-KO mice. This work was supported by the National Foundation of Natural Science of China (31100780 and 31200818), the Public Benefit Research Project of Zhejiang Province Department of Science and Technology (2013C33233), Seeds Fund for Interdisciplinary Research at Zhejiang University (JCZZ-2013037), and the Foundation of Zhejiang Educational Committee (Y201121662 and Y20070109).
Conflicts of Interest
There are no conflicts of interest for Li-Da Su, Ya-Jun Xie, Liang Zhou, Ying Shen, and Ying-Hong Hu.
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