The Cerebellum

, Volume 13, Issue 5, pp 607–615 | Cite as

Glutamate and GABA-Metabolizing Enzymes in Post-mortem Cerebellum in Alzheimer’s Disease: Phosphate-Activated Glutaminase and Glutamic Acid Decarboxylase

  • G. Sh. Burbaeva
  • I. S. Boksha
  • E. B. Tereshkina
  • O. K. Savushkina
  • T. A. Prokhorova
  • E. A. Vorobyeva
Original Paper

Abstract

Enzymes of glutamate and GABA metabolism in postmortem cerebellum from patients with Alzheimer’s disease (AD) have not been comprehensively studied. The present work reports results of original comparative study on levels of phosphate-activated glutaminase (PAG) and glutamic acid decarboxylase isoenzymes (GAD65/67) in autopsied cerebellum samples from AD patients and matched controls (13 cases in each group) as well as summarizes published evidence for altered levels of PAG and GAD65/67 in AD brain. Altered (decreased) levels of these enzymes and changes in links between amounts of these enzymes and other glutamate-metabolizing enzymes (such as glutamate dehydrogenase and glutamine synthetase-like protein) in AD cerebella suggest significantly impaired glutamate and GABA metabolism in this brain region, which was previously regarded as not substantially involved in AD pathogenesis.

Keywords

Phosphate-activated glutaminase Glutamic acid decarboxylase GABA metabolism Glutamate metabolism Alzheimer’s disease Autopsied cerebellum 

Abbreviations

AD

Alzheimer’s disease

APOE

Apolipoprotein E

AU

Arbitrary units

Amyloid beta peptide

CK BB

Creatine kinase BB

GABA

Gamma-aminobutyric acid

GAD

Glutamic acid decarboxylase

GDH

Glutamic acid dehydrogenase

Glu

Glutamic acid

Gln

Glutamine

GSLP

Glutamine synthetase-like protein

HRP

Horseradish peroxidase

PAG

Phosphate-activated glutaminase

PFC

Prefrontal cortex

PMI

Postmortem interval

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • G. Sh. Burbaeva
    • 1
  • I. S. Boksha
    • 2
  • E. B. Tereshkina
    • 1
  • O. K. Savushkina
    • 1
  • T. A. Prokhorova
    • 1
  • E. A. Vorobyeva
    • 1
  1. 1.Mental Health Research CenterRussian Academy of Medical SciencesMoscowRussia
  2. 2.Laboratory of Neurochemistry, Mental Health Research CenterRussian Academy of Medical SciencesMoscowRussia

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