Systemic mastocytosis associated with t(8;21)(q22;q22) acute myeloid leukemia
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Although KIT mutations are present in 20–25% of cases of t(8;21)(q22;q22) acute myeloid leukemia (AML), concurrent development of systemic mastocytosis (SM) is exceedingly rare. We examined the clinicopathologic features of SM associated with t(8;21)(q22;q22) AML in ten patients (six from our institutions and four from published literature) with t(8;21) AML and SM. In the majority of these cases, a definitive diagnosis of SM was made after chemotherapy, when the mast cell infiltrates were prominent. Deletion 9q was an additional cytogenetic abnormality in four cases. Four of the ten patients failed to achieve remission after standard chemotherapy and seven of the ten patients have died of AML. In the two patients who achieved durable remission after allogeneic hematopoietic stem cell transplant, recipient-derived neoplastic bone marrow mast cells persisted despite leukemic remission. SM associated with t(8;21) AML carries a dismal prognosis; therefore, detection of concurrent SM at diagnosis of t(8;21) AML has important prognostic implications.
KeywordsSystemic mastocytosis Acute myeloid leukemia KIT mutations Pathogenesis Translocation (8;21) Prognosis
The t(8;21)(q22;q22) occurs in about 5% of all acute myeloid leukemias (AML) and in 10% of cases of AML with FAB-M2 morphology . The Runt domain transcription factor RUNX1 (AML1) is essential for differentiation of hematopoietic stem cells. The t(8;21) generates the RUNX1-RUNX1T1 (AML1-ETO) fusion protein that acts as transcriptional repressor and inhibits the expression of AML1 responsive genes. This in turn causes a block in hematopoietic stem cell differentiation which in conjunction with additional genetic events leads to leukemic transformation [2, 3]. Studies have shown that t(8;21) by itself is incapable of leukemogenesis and additional genetic events are necessary for development of AML [4, 5]. Among these additional genetic changes that cooperate with RUNX1-RUNX1T1 fusion to cause AML, activating KIT mutations appear to be the most common. Data suggest that activating mutations in the receptor tyrosine kinases such as KIT play a role in the development of overt t(8;21)AML [5, 6, 7, 8].
Systemic mastocytosis (SM) occurs due to an activating KIT mutation in a hematopoietic progenitor that leads to abnormal proliferation and accumulation of neoplastic mast cells in the bone marrow and other organs. In a subset of SM termed systemic mastocytosis with associated hematological non-mast cell disease (SM-AHNMD) in the current WHO classification, SM coexists with hematologic malignancies that are usually of myeloid origin [1, 9]. SM-AHNMD comprises 20% of all cases of SM and the most common associated hematologic malignancies are acute myeloid leukemia (AML), myelodysplastic syndrome (MDS), chronic myeloproliferative disorders, and myelodysplastic/myeloproliferative syndromes such as chronic myelomonocytic leukemia [10, 11, 12, 13].
In previous studies, the frequency of KIT mutations involving exon 17 as represented by a single amino acid substitution at D816 in patients with t(8;21)(q22;q22) AML have ranged from 12% to 24.2% [14, 15, 16, 17]. Most common mutations described were at D816 and involved the Asp to Val substitution (D816V) [14, 15, 16, 17]. Other substitutions included D816H, D816Y, and those at N822 including N822K and N822T [14, 15, 16, 17]. Despite the fact that activating exon 17 KIT mutations are not uncommon in t(8;21) AML, a review of published literature suggests that SM associated with t(8;21) AML is exceedingly rare. Data on such cases are limited and largely confined to a handful of single case reports. We report our experience with SM associated with t(8;21)(q22;q22) AML and review the current literature on this rare entity.
Materials and methods
Patients with t(8;21) AML who also met WHO criteria for SM either at initial diagnosis or at relapse were identified from the databases of our institutions. Additional cases were identified by PubMed search of the English language literature as well as from the reference list of the reported cases [17, 18, 19, 20]. Immunohistochemistry for CD117, tryptase, and CD25 staining was performed using standard techniques. Polymerase chain reaction (PCR) amplification and direct sequencing of the exon 17 of the KIT gene were performed by methods previously described . Briefly, genomic DNA was isolated from bone marrow specimens using a QiAmp kit (Qiagen, Valencia, CA, USA). The codon 816-containing region of the KIT gene was amplified using the primers: 5′-TGT GAA CAT CAT TCA AGG CGT AC-3′ (forward) and 5′-ACT CAG CCT GTT TCT GGG AAA CTC-3′ (reverse). PCR conditions were each cycle of 30 s at 93°C, 1 min at 50°C, and 5 min at 72°C for a total of 40 cycles. The resulting 322-bp product was purified from a 2.5% agarose gel using a gel extraction kit (Qiagen) and directly sequenced. On case 3, fluorescence in situ hybridization (FISH) analysis for t(8;21)(q22;q22) on morphologically identified bone marrow mast cells (target FISH) was performed. Briefly, Wright-Giemsa stained bone marrow aspirate smears were scanned with a Bioview Duet Image Analyzer (Bioview, Rehovot, Israel) to identify mast cells for FISH. Following destaining with Carnoy’s fixative and digestion with pepsin, slides were co-denatured with t(8;21) probe (Vysis, Downer’s Grove, IL, USA). Slides were counterstained with DAPI and analyzed on a BioView Duet Image analyzer. Details of the target FISH technique were described previously .
Clinical and pathological features of SM with t(8;21) AML
Bone marrow morphology
KIT Exon 17
Case 1 
AML with multilineage dysplasia, WBC—35,400/µL 44% blasts
Hypercellular with Multilineage Dysplasia, 43% myeloblasts
CD13, CD33, CD11b, CD34, HLA-DR, CD56 CD7dim CD19 negative
Idarubicin + cytarabine followed by mitoxantrone + etoposide
Induction failure, died of progressive leukemia
Increased mast cells distributed randomly and perivascularly, indented nuclei, normal granularity
Case 2 
AML with multilineage dysplasia
Hypercellularity with multilineage dysplasia, 31% myeloblasts
CD13, CD33, CD34, CD117
Daunorubicin + cytarabine followed by HiDAC for 3 cycles
Remission achieved after induction. Relapsed with myeloid sarcoma 1 year after diagnosis. Underwent HSCT but died of relapsed disease 3 years later
Increased mast cells scattered and perivascular, normal morphology. Mast cell infiltrate prominent after treatment
Case 3 
40% myeloblasts, increased mast cells (30% of cellularity)
CD13, CD33CD, CD38, CD117, HLA-DR, MPO
Trytase, CD2, CD25
46X t(8;21)(q22;q22) del(9)(q12;q22) Monosomy X
Daunorubicin + cytarabine followed by 2 cycles of HiDAC
AML in complete remission after induction chemotherapy. Persistence of mast cells
Prominent infiltrate of clustered, spindle-shaped mast cells (80–90% of cellularity) after leukemia remission
Allogeneic HSCT from matched sibling after conditioning with TBI and etoposide. AML in remission 4 years after SCT. Persistence but gradual decline of recipient mast cells [carrying t (8;21)] 1 year after HSCT
1 year history of metastatic breast cancer treated with anthracycline-based chemotherapy. Therapy-related AML. WBC 58,200/µL, 96% blasts
Complete replacement of diagnosis bone marrow by myeloblasts. No increase in mast cells
CD34, CD33dim, CD117dim, HLA-DR, CD56, CD19 (dim)
Daunorubicin + cytarabine consolidation with HiDAC 3 cycles
Morphologic remission after induction. Bone marrow after consolidation showed relapse. Died of relapsed AML 8 months after diagnosis
70% cellular post-consolidation marrow with 27% blasts and numerous mast cells with indented twisted nuclei and normal granularity
FAB-M2 AML. WBC—45,400/µL
Hypercellular, 76% myeloblasts with increased mast cells, occasional spindled mast cells present
CD13, CD33 (dim), CD56 (bright), CD34, CD117, CD19 (dim)
CD2, CD25, Tryptase
45XY t(8;21)(q22;q22), del9(q22) 
Idarubicin + cytarabine
Induction failure. Died of progressive leukemia 1 year after diagnosis
FAB-M2 AML WBC 92,600/µL 82% blasts
95% cellularity, 60% myeloblasts, increased mast cells in perivascular and paratrabecular aggregates comprising about 20% of marrow cellularity. Pronounced mast cell infiltrate after treatment
CD13, CD56 CD15a, CD34, CD38 CD19a CD117, HLA-DR
46XX t(8;21)(q22;q22) del(9)(q22q34)
Idarubicin + cytarabine + Imatinib
Induction failure. Awaiting stem cell transplant
Wong et al. 
FAB-M2 AML WBC—2,800/µL 12% blasts
60% myeloblasts and increased mast cells positive for chloro-acetate esterase
MPO Chloroacetate esterase
t(8;21)(q22;q22) Monosomy 21
Danorubicin, thioguanine, cytarabine followed by cytarabine and mitoxantrone
Morphologic remission of leukemia after chemotherapy. Persistence of bone marrow mastocytosis and development of hepatosplenomegaly from mast cell infiltration. Died of disease 6 months after diagnosis
Escribano et al. 
FAB-M2 AML WBC-25,000/µL 71% blasts
Aspirate revealed 59% myeloblasts, no dysplasia. Increased atypical mast cells, oval nuclei, hypogranular cytoplasm and abnormal granularity
CD13, 33, 117 CD7a, CD19a
Tryptase CD2, 25, 117
Normal conventional cytogenetics (46XX), FISH: t(8;21)(q22;q22)
D816V not detected c
Idarubicin + cytarabine 2 cycles
Morphologic remission of AML after 2 cycles of induction. Persistent bone marrow mastocytosis. Relapsed 1 year after diagnosis and died of disease in second relapse 2 years after diagnosis
Bernd et al. 
Hypercellularity, sheets of myeloblasts
Myeloperoxidae and chloroacetate esterase
CD25, CD117, tryptase
Idarubicin + cytarabine for 3 cycles, HiDAC one cycle
Morphologic remission of AML after one cycle of chemotherapy. AML remained in remission 20 months after diagnosis. Bone marrow mastocytosis persisted for 18 months
Loosely scattered mast cells at diagnosis. Mastocytosis prominent after leukemic remission
Nagai et al. 
WBC—18,600/µL 69% blasts
Hypercellularity, 54% myeloblasts, multifocal dense infiltrates of mast cells at diagnosis. Mastocytosis pronounced after induction chemotherapy
Positive: CD13, CD34, CD56, CD117, HLA-DR
46XX, t(8;21)(q22;q22) del9 (q22;q34)
Idarubicin + cytarabine followed by mitoxantrone + cytarabine
Induction failure after 2 cycles of chemotherapy
Allogeneic HSCT from matched sibling after conditioning with TBI and cyclophosphamide
Persistent bone marrow mastocytosis but remission of leukemia over 1 year after HSCT
All patients were initially treated with AML induction chemotherapy that contained an anthracycline and cytarabine. Four of the ten patients failed to achieve a morphologic remission after induction chemotherapy. Six of the ten patients have died of progressive or relapsed leukemia and one patient remains with active disease awaiting allogeneic HSCT. The two long-term survivors (cases 3 and 10) in this report had both undergone allogeneic HSCT from matched siblings after radiation-based high-dose conditioning. In these two patients, recipient-derived bone marrow mast cells were detected up to a year after HSCT, but the leukemia remained in durable remission.
In this report, we have summarized our experience and reviewed the published literature on SM that coexists with t(8;21)(q22;q22) AML. KIT mutations are the most common additional genetic abnormality in t(8;21) AML and range in their incidence from 26% to 47% in various reports [22, 23, 24]. However, among the t(8;21) AML with KIT mutations, the number of cases that have concurrent SM appears to be extremely rare. Hence, it appears that specific genetic events in addition to activating KIT mutations and t(8;21) are required for development of mastocytosis. Although it can be postulated that these additional genetic events may promote mast cell differentiation from leukemic progenitors, the nature of these additional genetic aberrations remains unknown.
Studies examining the relationship between mast cells and leukemic blasts have shown that the neoplastic mast cells of SM associated with t(8;21) carry the RUNX1-RUNX1T1 translocation, thereby proving their derivation from the leukemic clone. This was demonstrated in case 3 of this series by target FISH, the details of which have been published elsewhere . Similar findings were noted in another published case that is included in the current series (case 10, Nagai et al.) where KIT mutation as well as RUNX1-RUNX1T1 translocation was detected in recipient-derived clonal mast cells that persisted after allogeneic HSCT . In both these cases, the recipient-derived mast cells gradually declined and the AML remained in remission. It is unclear if this phenomenon represents gradual apoptosis of these mast cells or progressive elimination of leukemic progenitors due to graft-versus-leukemia effect.
We sought to examine if there were other distinct cytogenetic or pathologic features that were associated with SM and t(8;21) AML. Deletion of chromosome 9q was an additional cytogenetic finding in four of the ten cases. Del (9q) has been previously reported as part of a complex phenotype in another case of t(8;21) positive myelomastocytic leukemia (AML with increased bone marrow mast cells not meeting criteria for SM) . In addition, del (9q) is the most common additional cytogenetic abnormality in t(8;21) AML and has been reported in 7–14% of pediatric cases and 9.7% of adult cases . In comparison, del (9q) appears to be more frequent in t(8;21) with associated SM, suggesting that this deletion may play a role in the pathogenesis of SM. Recently, TLE1 and TLE4 have been identified as critical genes in the commonly deleted 9q region in t(8;21) AML . In vitro experiments using Kasumi-1 cell line showed that these genes behave as tumor suppressors and knockdown of TLE1 or TLE4 increased the rate of cell division of the AML1-ETO expressing Kasumi-1 cell line while forced expression of either caused apoptosis and cell death .
The precise incidence of SM coexisting with t(8;21) AML is unknown. In previous reports, authors have cautioned that in some cases of AML with coexisting SM, the diagnosis of SM maybe missed on the initial bone marrow evaluation due to the excess numbers of blasts which mask the underlying mast cells and the tendency of mast cells to localize within stroma of bone marrow particles in aspirate smears . The bone marrow mast cell infiltrate appears to become more evident when the leukemic blasts decrease after therapy as was seen in this report (Fig. 1b). This prominence of the mast cell infiltrate after chemotherapy may also be due to the poor sensitivity of the mast cells to leukemia chemotherapy as evidenced by their persistence even after high-dose chemotherapy conditioning for allogeneic HSCT . Infiltration of extramedullary tissues with mast cells, as well as symptoms due to mast cell mediator release, appear to be distinctly uncommon in cases of SM associated with t(8;21) AML. This may be due to the lack of functionality of these neoplastic MC as a result of the leukemic aberrations they carry.
AML with t(8;21)(q22;q22) as the sole cytogenetic abnormality in general have a favorable outcome when treated with consolidation regimens containing high-dose cytarabine [29, 30]; however, studies have shown that only 50% of t(8;21) AML patients are alive at 5 years . D816 KIT mutations were detected in 10.5% of patients with t(8;21) AML in one study . The presence of KIT mutations (D816 and others) may explain the poor prognosis of a subset of t(8;21) patients as activating exon 17 KIT mutations have now been shown to be a major adverse prognostic factor for event-free and overall survival in t(8;21) AML [14, 15, 16, 17] and a predictor of higher relapse risk . The number of patients with mutant KIT who had SM in addition has not been reported in any of these studies, which may be an indicator of its extreme rarity. Interestingly, in previous studies it has been reported that 91–100% of t(8;21) AML patients with KIT mutations achieved complete remission after chemotherapy [15, 25]. However, in our current series induction failure occurred in four of ten patients. This observation again re-emphasizes the fact that SM associated with AML has a grave prognosis.
It is worth noting that of the ten patients (Table 1) only two patients achieved a durable remission of AML. Both of these patients had undergone allogeneic HSCT. One of our patients (case 3) is in continuous remission 4 years after allogeneic HSCT. Sperr et al. have reported a patient with myelomastocytic leukemia and t(8;21) AML who relapsed after a reduced intensity HSCT, but achieved a lasting remission following high-dose conditioning and a second HSCT from the same donor, thereby suggesting that conditioning intensity may be important . Although data are very limited, the dismal results with conventional chemotherapy suggests that allogeneic HSCT should be an early consideration in SM associated with t(8;21) AML in patients who are suitable candidates. The D816V KIT mutation is resistant to the tyrosine kinase inhibitor imatinib . Dasatinib, to which KIT D816V is sensitive, may have a role in the therapy of these cases as evidenced by in vitro data as well as its in vivo activity of this drug in recent reports of SM, including a patient with SM-AML [32, 33, 34]. Other tyrosine kinase inhibitors like midostaurin (PKC 412) and nilotinib (AMN 107)) that are capable of inhibiting activating KIT D816V may also have potential in the treatment of t(8;21) AML that carry this mutation [35, 36].
In conclusion, SM associated with t(8;21) AML is extremely rare and carries a dismal prognosis. During evaluation of t(8;21)(q22;q22) AML, care should be taken to look for coexisting SM in the initial and subsequent bone marrow specimens since the mast cell infiltrate may be subtle and easily overlooked. This will help identify a subset of t(8;21) AML patients with a particularly poor prognosis.
The authors declare that there are no sources of support to acknowledge.
Conflicts of interest statement