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Cancer Microenvironment

, Volume 11, Issue 2–3, pp 115–124 | Cite as

Ascites IL-10 Promotes Ovarian Cancer Cell Migration

  • Denis Lane
  • Isabelle Matte
  • Perrine Garde-Granger
  • Paul Bessette
  • Alain PichéEmail author
Original Article
  • 98 Downloads

Abstract

Ovarian cancer (OC) ascites is an inflammatory and immunosuppressive tumor environment characterized by the presence of various cytokines, chemokines and growth factors. The presence of high concentrations of these cytokines/chemokines in ascites is associated with a more aggressive tumor phenotype. IL-10 is an immunosuppressive cytokine for which high expression has been associated with poor prognosis in some cancers. However, its role on OC tumor cells has not been explored. Therefore, the aim of the current study was to elucidate the role of ascites IL-10 on the proliferation, migration and survival of OC cell lines. Here, we show that IL-10 levels are markedly increased in patients with advanced serous OC ascites relative to serous stage I/II ascites and peritoneal effusions from women with benign conditions. Ascites and IL-10 dose-dependently enhanced the proliferation and migration of OC cell lines CaOV3 and OVCAR3 but had no effect on cell survival. IL-10 levels in ascites positively correlated with the ability of ascites to promote cell migration but not proliferation. Depletion of IL-10 from ascites markedly inhibited ascites-induced OC cell migration but was not crucial for ascites-mediated cell proliferation. Taken together, our findings establish an important role for IL-10, as a component of ascites, in the migration of tumor cells.

Keywords

Ovarian carcinoma Ascites IL-10 Cell migration Cell proliferation 

Notes

Acknowledgements

This work was supported by funds from the Canadian Institute for Health Research (MOP-244194-CPT-CFDA-48852), the Centre de Recherche Clinique Étienne-Lebel du Centre Hospitalier Universitaire de Sherbrooke and the Université de Sherbrooke. Tumor banking were supported by the Banque de tissus et données of the Réseau de recherche sur le cancer of the Fond de recherche du Québec en Santé (FRQS) and Cancer de l’Ovaire Canada, associated with the Canadian Tumor Repository Network (CTRNet). The tissue bank provided malignant ascites and peritoneal fluids.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no competing interests.

Supplementary material

12307_2018_215_MOESM1_ESM.ppt (266 kb)
ESM 1 (PPT 265 kb)

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Denis Lane
    • 1
  • Isabelle Matte
    • 1
  • Perrine Garde-Granger
    • 2
  • Paul Bessette
    • 3
  • Alain Piché
    • 1
    Email author
  1. 1.Département de Microbiologie et InfectiologieUniversité de SherbrookeSherbrookeCanada
  2. 2.Département de PathologieUniversité de SherbrookeSherbrookeCanada
  3. 3.Service d’obstétrique et gynécologie, Département de Chirurgie, Faculté de MédecineUniversité de SherbrookeSherbrookeCanada

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