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Influence of different seed priming techniques on oxidative and antioxidative responses during the germination of Oryza sativa varieties

Abstract

NaCl and PEG stresses have negative impacts on seed germination and early seedling establishment in Oryza sativa. The present study was designed to ascertain the influence of different priming techniques (Hydro priming-HyP, Halo priming-HP, UV-B priming-UP) in enhancing oxidative and anti-oxidative mechanisms during seed germination phase in response to NaCl and PEG stresses tolerance of three rice varieties (Neeraja, Vaisakh and Vyttila 6). NaCl and PEG stresses caused delayed germination rate, enhanced reactive oxygen species content and thereby increased lipid peroxidation rate. Different priming techniques significantly hastened the metabolites/non enzymatic antioxidant contents (total sugars, total phenolics, free amino acids, proline, ascorbate and glutathione) as well as activities of antioxidant enzymes (superoxide dismutase, catalase, ascorbate peroxidase and guaiacol peroxidase), and thus reduced oxidative stress damages caused by NaCl and PEG stresses in rice seedlings. Seed priming techniques imparted abiotic stress tolerance not only to sensitive varieties but also additional tolerance potential to tolerant varieties. All three priming techniques protects the plants from toxicity caused by NaCl and PEG stresses but halo priming had proved to be more successful.

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Acknowledgements

AS is indebted to the Council of Scientific & Industrial Research (CSIR), New Delhi, for the financial assistance through award of Junior Research Fellowship (JRF). The Regional Agricultural Research Station (RARS) Pattambi and Vyttila, Kerala, India, is gratefully acknowledged for providing seeds of rice varieties. We thank Dinakar Challabathula, Department of Life Sciences, Central University of Tamil Nadu, India for helpful discussion and suggestions related to the manuscript.

Author information

AS wrote the main text of the manuscript and collected the necessary references. JTP edited the manuscript. All authors have read and approved the final manuscript.

Correspondence to Jos T. Puthur.

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Sen, A., Puthur, J.T. Influence of different seed priming techniques on oxidative and antioxidative responses during the germination of Oryza sativa varieties. Physiol Mol Biol Plants (2020). https://doi.org/10.1007/s12298-019-00750-9

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Keywords

  • Seed priming
  • NaCl stress
  • PEG stress
  • Hydro priming
  • Halo priming
  • UV-B priming