Genetic diversity and population structure detection in sponge gourd (Luffa cylindrica) using ISSR, SCoT and morphological markers

  • Ruchi Tyagi
  • Vinay Sharma
  • Amish Kumar Sureja
  • Anilabh Das Munshi
  • Lalit Arya
  • Dipnarayan Saha
  • Manjusha VermaEmail author
Research Article


Investigation of genetic diversity is essential for the selection of parents for crop breeding and conservation of genetic resources. To estimate the genetic variability and population structure in the midst of 45 accessions of sponge gourd brought together from different geographical areas of India, morphological traits and two molecular markers, ISSR and SCoT markers were compared. Principal components analysis of 20 morphological traits showed 72.70% variability and significant positive correlations between fruit traits. All three marker techniques clustered all accessions into two groups with few outgroups. High level of polymorphism was observed among ISSR (74.6%) and SCoT (71.5%) primers. The Bayesian model revealed the hidden grouping and showed admixture type of population. The diversity pattern is influenced by genetic marker used, as different molecular markers have different polymorphism evaluation efficiency. This study can be helpful in amplifying the genetic base and selection of specific traits for breeding. Thus, ISSR and SCoT markers are potential marker for identification in sponge gourd and provide valuable data on its genetic correlation and structure.


Luffa cylindrica ISSR SCoT Morphological marker Genetic variability Sponge gourd Population structure 



Un-weighted pair group method with arithmetic average


Inter simple sequences repeat


Polymorphism information content


Principal component analysis


Start codon targeted


Polymerase chain reaction



We are grateful to the Head, Division of Genomic Resources and the Director, National Bureau of Plant Genetic Resources for providing access to the facilities.


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Copyright information

© Prof. H.S. Srivastava Foundation for Science and Society 2019

Authors and Affiliations

  • Ruchi Tyagi
    • 1
  • Vinay Sharma
    • 1
  • Amish Kumar Sureja
    • 2
  • Anilabh Das Munshi
    • 2
  • Lalit Arya
    • 3
  • Dipnarayan Saha
    • 4
  • Manjusha Verma
    • 3
    Email author
  1. 1.Department of Bioscience and BiotechnologyBanasthali UniversityBanasthaliIndia
  2. 2.Division of Vegetable ScienceIndian Agricultural Research InstituteNew DelhiIndia
  3. 3.Division of Genomic ResourcesICAR-NBPGRNew DelhiIndia
  4. 4.ICAR-Central Research Institute for Jute and Allied Fibres (ICAR)BarrackporeIndia

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