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Physiology and Molecular Biology of Plants

, Volume 25, Issue 6, pp 1349–1366 | Cite as

Groundnut AhcAPX conferred abiotic stress tolerance in transgenic banana through modulation of the ascorbate–glutathione pathway

  • Shashi Shekhar
  • Anjana Rustagi
  • Deepak Kumar
  • Mohd. Aslam Yusuf
  • Neera Bhalla SarinEmail author
  • Kapil Lawrence
Research Article
  • 22 Downloads

Abstract

A stress inducible cytosolic ascorbate peroxidase gene (AhcAPX) was ectopically expressed in banana (cv. Grand naine) plants to strengthen their antioxidant capacity. High level of AhcAPX gene transcripts and enzyme suggested constitutive and functional expression of candidate gene in transgenic (TR) plants. The tolerance level of in vitro and in vivo grown TR banana plantlets were assessed against salt and drought stress. The TR banana plants conferred tolerance against the abiotic stresses by maintaining a high redox state of ascorbate and glutathione, which correlated with lower accumulation of H2O2, O 2 ⋅− and higher level of antioxidant enzyme (SOD, APX, CAT, GR, DHAR and MDHAR) activities. The efficacy of AhcAPX over-expression was also investigated in terms of different physiochemical attributes of TR and untransformed control plants, such as, proline content, membrane stability, electrolyte leakage and chlorophyll retention. The TR plants showed higher photochemical efficiency of PSII (Fv/Fm), and stomatal attributes under photosynthesis generated reactive oxygen species (ROS) stress. The outcome of present investigation suggest that ectopic expression of AhcAPX gene in banana enhances the tolerance to drought and salt stress by annulling the damage caused by ROS.

Keywords

Abiotic stress Musa Cytosolic ascorbate peroxidase (cAPX) Stomata Photosynthesis 

Notes

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Copyright information

© Prof. H.S. Srivastava Foundation for Science and Society 2019

Authors and Affiliations

  • Shashi Shekhar
    • 1
    • 2
  • Anjana Rustagi
    • 3
  • Deepak Kumar
    • 4
  • Mohd. Aslam Yusuf
    • 5
  • Neera Bhalla Sarin
    • 2
    Email author
  • Kapil Lawrence
    • 1
  1. 1.Department of Biochemistry and Biochemical Engineering, Jacob School of Biotechnology and BioengineeringSam Higginbottom University of Agriculture Technology and SciencesAllahabadIndia
  2. 2.School of Life SciencesJawaharlal Nehru UniversityNew DelhiIndia
  3. 3.Department of Botany, Gargi CollegeUniversity of DelhiNew DelhiIndia
  4. 4.Department of BotanyCentral University of JammuJammuIndia
  5. 5.Department of BioengineeringIntegral UniversityLucknowIndia

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