The effects of the electromagnetic fields on the biochemical components, enzymatic and non-enzymatic antioxidant systems of tea Camellia sinensis L. Research Article First Online: 27 August 2019 Abstract
The electromagnetic fields (EMFs) by wide range of the frequency spectrum, have capability to cause crucial alternation and deleterious effects in biological systems. The aim of the present study is to assay the biochemical components, enzymatic and non-enzymatic antioxidant systems of the electromagnetic fields treated samples of tea which is the most ancient non-alcoholic drink, containing different types of flavonols. Rutin, Quercetin, Myricetin, and Kaempferol as flavonoid components markers are also to be analyzed using high-performance liquid chromatography. The results show that The EMF’s treatments brought about distinct alternations in biochemical components of tea, so that regardless of the intensity of the EMF’s, less duration of exposure (30 min) caused more content of those mentioned flavonoid components (except Myricetin) than that of 60 min of exposure. A 30 min of 4 miliTesla (mT) exposure of the EMF’s, resulted in the highest amount of Rutin, Quercetin, Myricetin, and Kaempferol. It is concluded that less duration of the EMF’s treatments induces more production and also accumulation of enzymatic and non-enzymatic antioxidant components. In higher intensity of the EMF’s (more than 4 mT), the concentrations of the mentioned biochemical components decreased.
Keywords Electromagnetic fields Enzymatic and nonenzymatic antioxidant Tea Camellia sinensis L. HPLC Flavonoid and polyphenols Notes Funding
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Ahmad P, Jaleel CA, Salem MA, Nabi G, Sharma S (2010) Roles of enzymatic and nonenzymatic antioxidants in plants during abiotic stress. Crit Rev Biotechnol 30:161–175.
https://doi.org/10.3109/07388550903524243 CrossRef PubMed Google Scholar
Asghar T, Jamil Y, Iqbal M, Zia-ul-Haq Abbas M (2016) Laser light and magnetic field stimulation effect on biochemical, enzymes activities and chlorophyll contents in soybean seeds and seedlings during early growth stages. J Photochem Photobiol B 165:283–290.
https://doi.org/10.1016/j.jphotobiol.2016.10.022 CrossRef PubMed Google Scholar
Astaneh RK, Bolandnazar S, Nahandi FZ, Oustan S (2018) Effect of selenium application on phenylalanine ammonia-lyase (PAL) activity, phenol leakage and total phenolic content in garlic (
L.) under NaCl stress information. Process Agric 5:339–344.
https://doi.org/10.1016/j.inpa.2018.04.004 CrossRef Google Scholar
Atak C, Emirolu Ö, Alikamanolu S (2003) Stimulation of regeneration by magnetic field in soybean (
L. Merrill) tissue cultures. J Cell Mol Biol 2:113–119
Bagheri Abyaneh E (2018) Low frequency electromagnetic field induced oxidative stress in
L. Iran J Sci Technol Trans A Sci 42:1419–1426.
https://doi.org/10.1007/s40995-016-0105-9 CrossRef Google Scholar
Bennett RN, Wallsgrove RM (1994) Secondary metabolites in plant defence mechanisms. New Phytol 127:617–633.
https://doi.org/10.1111/j.1469-8137.1994.tb02968.x CrossRef Google Scholar
Büyükuslu N, Çelik Ö, Atak C (2006) The effect of magnetic field on the activity of superoxide dismutase. J Cell Mol Biol 5:57–62
Casati P, Walbot V (2003) Gene expression profiling in response to ultraviolet radiation in maize genotypes with varying flavonoid content. Plant Physiol 132:1739–1754.
https://doi.org/10.1104/pp.103.022871 CrossRef PubMed PubMedCentral Google Scholar
Cebulak T, Oszmiański J, Kapusta I, Lachowicz S (2017) Effect of UV-C Radiation, ultra-sonication electromagnetic field and microwaves on changes in polyphenolic compounds in chokeberry (
). Molecules 22:1161.
https://doi.org/10.3390/molecules22071161 CrossRef PubMedCentral Google Scholar
Davidson VL (2010) 7.19 - protein-derived cofactors. In: Liu H-W, Mander L (eds) Comprehensive natural products II. Elsevier, Oxford, pp 675–710.
https://doi.org/10.1016/B978-008045382-8.00143-X CrossRef Google Scholar
Del Rio D, Stewart AJ, Mullen W, Burns J, Lean MEJ, Brighenti F, Crozier A (2004) HPLC-MSn analysis of phenolic compounds and purine alkaloids in green and black tea. J Agric Food Chem 52:2807–2815.
https://doi.org/10.1021/jf0354848 CrossRef PubMed Google Scholar
Elavarthi S, Martin B (2010) Spectrophotometric assays for antioxidant enzymes in plants. In: Sunkar R (ed) Plant stress tolerance: methods and protocols. Humana Press, Totowa, pp 273–280.
https://doi.org/10.1007/978-1-60761-702-0_16 CrossRef Google Scholar
Freitas AMB, Landgraf F, Nvltý J, Giulietti M (1999) Influence of magnetic field in the kinetics of crystallization of diamagnetic and paramagnetic inorganic salts. Cryst Res Technol 34:1239–1244.
https://doi.org/10.1002/(sici)1521-4079(199912)34:10%3c1239:aid-crat1239%3e3.0.co;2-9 CrossRef Google Scholar
Iqbal M, Haq Zu, Jamil Y, Nisar J (2016) Pre-sowing seed magnetic field treatment influence on germination, seedling growth and enzymatic activities of melon (
L.). Biocatal Agric Biotechnol 6:176–183.
https://doi.org/10.1016/j.bcab.2016.04.001 CrossRef Google Scholar
Kataria S, Baghel L, Guruprasad K (2017) Pre-treatment of seeds with static magnetic field improves germination and early growth characteristics under salt stress in maize and soybean. Biocatal Agric Biotechnol 10:83–90.
https://doi.org/10.1016/j.bcab.2017.02.010 CrossRef Google Scholar
Kataria S, Baghel L, Jain M, Guruprasad KN (2019) Magnetopriming regulates antioxidant defense system in soybean against salt stress. Biocatal Agric Biotechnol 18:101090.
https://doi.org/10.1016/j.bcab.2019.101090 CrossRef Google Scholar
Kim DS, Hwang BK (2014) An important role of the pepper phenylalanine ammonia-lyase gene (PAL1) in salicylic acid-dependent signalling of the defence response to microbial pathogens. J Exp Bot 65:2295–2306.
https://doi.org/10.1093/jxb/eru109 CrossRef PubMed PubMedCentral Google Scholar
Kıvrak EG, Yurt KK, Kaplan AA, Alkan I, Altun G (2017) Effects of electromagnetic fields exposure on the antioxidant defense system. J Microsc Ultrastruct 5:167–176.
https://doi.org/10.1016/j.jmau.2017.07.003 CrossRef PubMed PubMedCentral Google Scholar
Kumar S, Pandey AK (2013) Chemistry and biological activities of flavonoids: an overview. Sci World J 2013:16.
https://doi.org/10.1155/2013/162750 CrossRef Google Scholar
Li Y, Ma D, Sun D, Wang C, Zhang J, Xie Y, Guo T (2015) Total phenolic, flavonoid content, and antioxidant activity of flour, noodles, and steamed bread made from different colored wheat grains by three milling methods. Crop J 3:328–334.
https://doi.org/10.1016/j.cj.2015.04.004 CrossRef Google Scholar
Mondal TK (2014) Breeding and biotechnology of tea and its wild species. Springer, India
CrossRef Google Scholar
Nakano Y, Asada K (1987) Purification of ascorbate peroxidase in spinach chloroplasts; its inactivation in ascorbate-depleted medium and reactivation by monodehydroascorbate. Radic Plant Cell Physiol 28:131–140.
https://doi.org/10.1093/oxfordjournals.pcp.a077268 CrossRef Google Scholar
Parola A, Kost D, Katsir G, Ben-Izhak Monselise E, Cohen-Luria R (2005) Radical scavengers suppress low frequency EMF enhanced proliferation in cultured cells and stress effects in higher plants. Environmentalist 25:103–111.
https://doi.org/10.1007/s10669-005-4272-z CrossRef Google Scholar
Petrussa E, Braidot E, Zancani M, Peresson C, Bertolini A, Patui S, Vianello A (2013) Plant flavonoids–biosynthesis, transport and involvement in stress responses. Int J Mol Sci 14:14950–14973.
https://doi.org/10.3390/ijms140714950 CrossRef PubMed PubMedCentral Google Scholar
Piacentini MP, Fraternale D, Piatti E, Ricci D, Vetrano F, Dachà M, Accorsi A (2001) Senescence delay and change of antioxidant enzyme levels in
L. etiolated seedlings by ELF magnetic fields. Plant Sci 161:45–53.
https://doi.org/10.1016/S0168-9452(01)00380-6 CrossRef Google Scholar
Radhakrishnan R, Ranjitha Kumari BD (2012) Pulsed magnetic field: a contemporary approach offers to enhance plant growth and yield of soybean. Plant Physiol Biochem 51:139–144.
https://doi.org/10.1016/j.plaphy.2011.10.017 CrossRef PubMed Google Scholar
Schroeter H, Boyd C, Spencer JPE, Williams RJ, Cadenas E, Rice-Evans C (2002) MAPK signaling in neurodegeneration: influences of flavonoids and of nitric oxide. Neurobiol Aging 23:861–880.
https://doi.org/10.1016/S0197-4580(02)00075-1 CrossRef PubMed Google Scholar
Scott IM, Clarke SM, Wood JE, Mur LAJ (2004) Salicylate accumulation inhibits growth at chilling temperature in arabidopsis. Plant Physiol 135:1040.
https://doi.org/10.1104/pp.104.041293 CrossRef PubMed PubMedCentral Google Scholar
Shabrangy A, Majd A (2009) Effect of magnetic fields on growth and antioxidant systems in agricultural plants. PIERS Proc 2:23–27
Shashurin MM, Prokopiev IA, Filippova GV, Zhuravskaya AN, Korsakov AA (2017) Effect of extremely low frequency magnetic fields on the seedlings of wild plants growing in Central Yakutia. Russ J Plant Physiol 64:438–444.
https://doi.org/10.1134/s1021443717030165 CrossRef Google Scholar
Shi Q, Zhu Z (2008) Effects of exogenous salicylic acid on manganese toxicity, element contents and antioxidative system in cucumber. Environ Exp Bot 63:317–326.
https://doi.org/10.1016/j.envexpbot.2007.11.003 CrossRef Google Scholar
Shine MB, Guruprasad KN (2012) Impact of pre-sowing magnetic field exposure of seeds to stationary magnetic field on growth, reactive oxygen species and photosynthesis of maize under field conditions. Acta Physiol Plant 34:255–265.
https://doi.org/10.1007/s11738-011-0824-7 CrossRef Google Scholar
Trebbi G, Borghini F, Lazzarato L, Torrigiani P, Calzoni GL, Betti L (2007) Extremely low frequency weak magnetic fields enhance resistance of NN tobacco plants to tobacco mosaic virus and elicit stress-related biochemical activities. Bioelectromagnetics 28:214–223.
https://doi.org/10.1002/bem.20296 CrossRef PubMed Google Scholar
Valifard M, Mohsenzadeh S, Niazi A, Moghadam A (2015) Phenylalanine ammonia lyase isolation and functional analysis of phenylpropanoid pathway under salinity stress in Salvia species. AJCS 9(7):656–665
Vian A, Davies E, Gendraud M, Bonnet P (2016) Plant responses to high frequency electromagnetic fields. Biomed Res Int 2016:1830262.
https://doi.org/10.1155/2016/1830262 CrossRef PubMed PubMedCentral Google Scholar
Volkov AG (2007) Plant electrophysiology: theory and methods. Springer, Berlin
Wada KC, Mizuuchi K, Koshio A, Kaneko K, Mitsui T, Takeno K (2014) Stress enhances the gene expression and enzyme activity of phenylalanine ammonia-lyase and the endogenous content of salicylic acid to induce flowering in pharbitis. J Plant Physiol 171:895–902.
https://doi.org/10.1016/j.jplph.2014.03.008 CrossRef PubMed Google Scholar
Wu W, Wan X, Shah F, Fahad S, Huang J (2014) The role of antioxidant enzymes in adaptive responses to sheath blight infestation under different fertilization rates and hill densities. Sci World J 2014:502134.
https://doi.org/10.1155/2014/502134 CrossRef Google Scholar Copyright information
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