Characterization of genetic diversity and population structure of Moroccan lentil cultivars and landraces using molecular markers
- 494 Downloads
Knowledge of genetic diversity and population structure is a crucial step for an efficient use of available material in a plant breeding program and for germplasm conservation strategies. Current study undertakes an assessment of the genetic variations and population structure of Moroccan lentil including nine landraces and eight released varieties using sequence-related amplified polymorphism (SRAP) and random amplified polymorphism DNA (RAPD) markers. Results revealed that the two markers used have a good efficiency to assess genetic diversity in lentil. A total of 115 and 90 bands were respectively scored for SRAP and RAPD, of which 98.3% and 93.3% were polymorphic. The polymorphic information content values were 0.350 with SRAP and 0.326 with RAPD. Analysis of molecular variance based on the combined data sets of both markers revealed lower variations within (35%) than among (65%) landraces (PhiPT = 0.652), implying significant genetic differentiation between landraces. Principal coordinate analysis and the ascendant hierarchical classification clustered samples into groups that were consistent with the geographical origin of the cultivars. Population structure corroborated the main groups and confirmed the high differentiation among them. Moroccan lentil germplasm showed a wide genetic diversity that might be conserved and assessed for tolerance to biotic and abiotic stresses.
KeywordsGenetic diversity Lens culinaris Medik. Morocco RAPD SRAP Population structure
This research was supported by National Institute of Agricultural Research (INRA-Morocco) and Ministry of Higher Education, Scientific Research and Professional Training of Morocco (MESRSFC) through funding of MEDILEG Project within the European Union 7th Framework program for research, technological development and demonstration (ERA-Net Project, ARIMNet).
Compliance with ethical standards
Conflicts of interest
The authors declare no conflicts of interest.
- Abd El Moneim AM, Ryan J (2004) Forage legumes for dryland agriculture in Central and West Asia and North Africa. In: Rao, Ryan J (eds) Challenges and strategies of dryland agriculture, crop science. Society of America and American Society of Agronomy, Madison, pp 243–256Google Scholar
- Alghamdi SS, Khan AM, Ammar MH, El-Harty EH, Migdadi HM, El-Khalik SM, Al-Shameri AM, Javed MM, Al-Faifi SA (2014) Phenological, nutritional and molecular diversity assessment among 35 introduced lentil (Lens culinaris Medik.) genotypes grown in Saudi Arabia. Int J Mol Sci 15:277–295CrossRefGoogle Scholar
- Arber W, Illmensee K, Peacock WJ, Starlinger P (1984) Genetic manipulation: impact on man and society. Cambridge University Press, CambridgeGoogle Scholar
- Benbrahim N, Taghouti M, Zouahri A, Gaboun F (2017) On-farm conservation of Zaer lentil landrace in context of climate change and improved varieties competition. Univ J Agric Res 5:27–38Google Scholar
- Bermejo C, Gatti I, Caballero N, Cravero V, Martin E, Cointry E (2014) Study of diversity in a set of lentil RILs using morphological and molecular markers. Aust J Crop Sci 8:689–696Google Scholar
- Devendra C (1997) Crop residues for feeding animals in Asia: technology development and adoption in crop/livestock systems. CAB International, WallingfordGoogle Scholar
- Idrissi O, Udupa SM, Houasli C, De Keyser E, Van Damme P, De Riek J (2015) Genetic diversity analysis of Moroccan lentil (Lens culinaris Medik.) landraces using Simple Sequence Repeat and amplified fragment length polymorphisms reveals functional adaptation towards agro-environmental origins. Plant Breed 134:322–332CrossRefGoogle Scholar
- Idrissi O, Udupa MS, De Keyser E, Van Damme P, De Riek J (2016) Functional genetic diversity analysis and identification of associated simple sequence repeats and amplified fragment length polymorphism markers to drought tolerance in lentil (Lens culinaris ssp. culinaris Medicus) landraces. Plant Mol Biol Rep 34:659–680CrossRefGoogle Scholar
- Keify F, Beiki AH (2012) Exploitation of random amplified polymorphic DNA (RAPD) and sequence-related amplified polymorphism (SRAP) markers for genetic diversity of saffron collection. J Med Plants Res 6:2761–2768Google Scholar
- Lardy G, Anderson V (2009) Alternative feeds for ruminants. NDSU, FargoGoogle Scholar
- Prakesh V, Tandon J, Prasad K (1986) Studies on intercropping rainfed wheat. Ann Agric Sci 7:258–262Google Scholar
- Shah Z, Shah SH, Peoples MB, Schwenke GD, Herridge DF (2003) Crop residue and fertiliser N effects on nitrogen fixation and yields of legume—cereal rotations and soil organic fertility. Front Plant Sci 83:1–11Google Scholar
- XLSTAT (2017) Data analysis and statistical solution for microsoft excel. Addinsoft, ParisGoogle Scholar