Accumulation of amino acids and flavonoids in hairy root cultures of common buckwheat (Fagopyrum esculentum)

  • Ahmed M. M. GabrEmail author
  • Oksana SytarEmail author
  • Hassan Ghareeb
  • Marian Brestic
Research Article


Transformed hairy root culture in common buckwheat (Fagopyrum esculentum Moench Rubra cultivar) was investigated for accumulation of amino acids and specific flavonoids. Leaves and stems of F. esculentum were used a starting material for induction of hairy roots via the Agrobacterium rhizogenes A4 strain. The transformed lines were confirmed by PCR detection of rol B gene, and their capability to continuously form hairy roots. Three lines from each explant types depending upon growth kinetics were observed. The hairy root lines were used to measure the contents of 17 amino acids and 3 flavonoids. Overall, the hairy root lines exhibited elevated accumulation of semi-essential amino acids such as lysine, isoleucine, valine, histidine and phenylalanine. Content of proline was increased 3–5 times, likely due to the biotic stress reaction induced with A. rhizogenes. Determination of flavonoids by high-performance liquid chromatography, hesperidine and kaempferol-3-rutinoside, were accumulated in hairy root cultures and didn’t detected in non-transformed root. The increase in flavonoids positively correlated with the antioxidant capacity of the hairy root cultures.


Common Buckwheat Agrobacterium rhizogenes Semi-essential amino acids Flavonoids 



Funding was provided by Agentúra na Podporu Výskumu a Vývoja (Grant No. APVV-15-0721).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Prof. H.S. Srivastava Foundation for Science and Society 2019

Authors and Affiliations

  1. 1.Department of Plant Biotechnology, Genetic Engineering and Biotechnology Research DivisionNational Research Centre (NRC)CairoEgypt
  2. 2.Plant Physiology and Ecology DepartmentTaras Shevchenko National University of Kyiv, Institute of BiologyKievUkraine
  3. 3.Departament of Plant PhysiologySlovak Agricultural University in NitraNitraSlovak Republic

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