Physiology and Molecular Biology of Plants

, Volume 25, Issue 1, pp 71–83 | Cite as

Differential response of Indian mustard (Brassica juncea L., Czern and Coss) under salinity: photosynthetic traits and gene expression

  • Jogendra Singh
  • Vijayata Singh
  • T. V. Vineeth
  • Parveen Kumar
  • Neeraj Kumar
  • Parbodh C. SharmaEmail author
Research Article


To explore the effect of salt stress on photosynthetic traits and gene expression in Indian mustard, four genotypes CS 54 (national check for salinity), CS 52-SPS-1-2012 (salt tolerant mutant), CS 614-4-1-4-100-13 (salt sensitive mutant) and Pusa bold (high yielding variety) were evaluated under irrigation water salinity (ECiw 12, and 15 dS m−1). Results suggest genotype CS 52-SPS-1-2012 followed by CS 54 performed better under imposed salt stress due to differential regulation of Na+ accumulation in the roots and main stem, restriction of Na+ influx from root to shoot, maintaining higher net photosynthetic traits under saline stress compared to CS 614-4-1-4-100-13 and Pusa bold. Further, overexpression of antiporters (SOS1, SOS2, SOS3, ENH1 and NHX1) and antioxidant (APX1, APX4, DHAR1 and MDHAR) genes in salt tolerant genotypes CS 52-SPS-1-2012 and CS 54 demonstrated their significant role in imparting salt tolerance in Indian mustard.


Salt tolerant Salinity Gene expression Photosynthetic traits 



Rate of photosynthesis (µmol CO2 m−2 s−1)


Stomatal conductance (mmol m−2 s−1)


Rate of transpiration (mmol H2O m−2 s−1)


Instantaneous water use efficiency [µmol (CO2) mmol−1 (H2O)]


CO2 assimilation (µmol CO2 mol−1)


Photosynthetically active radiation


Salt overly sensitive


Enhancer of SOS


Vacuolar Na+/H+ antiporters


Ascorbate peroxidase


Dehydroascorbate reductase


Mono-dehydroascorbate reductase


Compliance with ethical standards

Conflict of interest

Authors have no conflict of interest.

Supplementary material

12298_2018_631_MOESM1_ESM.docx (15 kb)
Supplementary material 1 (DOCX 15 kb)


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Copyright information

© Prof. H.S. Srivastava Foundation for Science and Society 2018

Authors and Affiliations

  • Jogendra Singh
    • 1
  • Vijayata Singh
    • 1
  • T. V. Vineeth
    • 1
  • Parveen Kumar
    • 1
  • Neeraj Kumar
    • 1
  • Parbodh C. Sharma
    • 1
    Email author
  1. 1.ICAR-Central Soil Salinity Research InstituteKarnalIndia

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