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Profiling of indole metabolic pathway in thermo-sensitive Bainong male sterile line in wheat (Triticum aestivum L.)

  • Qing Su
  • Jing Yang
  • Qing Yun Fu
  • Fei Yun Jia
  • Suo Ping Li
  • Yong Li
  • You Yong Li
Research Article
  • 21 Downloads

Abstract

Bainong male sterile (BNS) wheat (Triticum aestivum L.) is a thermo-sensitive genic male sterile line with excellent sterility and self-restoration. We focused on transcriptional profiles of differentially expressed probes between BNS sterile and fertile anthers. Anthers, rachis and spikes from sterile line and fertile line were collected. Extracted RNA was assayed using wheat expression microarray and Gene Ontology was analyzed using Cytoscape with ClueGO. An indole (indole-3-acetic acid: IAA) metabolism pathway sub-network was almost formed in all differentially expressed profiles between sterile and fertile samples. IAA sub-network contained four nodes of indole and alkaloid metabolism connecting main network via indole compounds. This sub-network was absent in rachis and intact in transformed fertile anthers, which was the main differently expressed metabolism pathway in F1 anthers with restorer genes. Alkaloid metabolism was absent in sterile anthers. Abnormal metabolism of IAA may be involved in BNS sterility. BNS transformation may be regulated by the production of IAA and alkaloid metabolism pathway, which favor the safe utilization of the sterile line in hybrid wheat production.

Keywords

Alkaloid metabolism BNS male sterility Gene expression microarray IAA metabolism Wheat 

Notes

Acknowledgements

This research was funded by Henan Research Project on Foundation and Frontier Technology (Nos. 122300410011 and 162300410136). We would like to thank prof. ZhenGang Ru, Directorate of Wheat Research Center in Henan institute of science and technology, for supply wheat BNS sterile line. We would also like to thank prof. Gonmbia who give many suggestions for ClueGO running.

Compliance with ethical standards

Conflict of interest

The authors have no conflict of interest to declare.

Supplementary material

12298_2018_626_MOESM1_ESM.doc (40 kb)
Supplementary material 1 (DOC 39 kb)

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Copyright information

© Prof. H.S. Srivastava Foundation for Science and Society 2018

Authors and Affiliations

  • Qing Su
    • 1
    • 2
  • Jing Yang
    • 1
  • Qing Yun Fu
    • 1
  • Fei Yun Jia
    • 1
  • Suo Ping Li
    • 2
  • Yong Li
    • 1
    • 3
  • You Yong Li
    • 1
  1. 1.Henan Institute of Science and Technology/Collaborative Innovation Center of Modern Biological BreedingXinxiangChina
  2. 2.Henan UniversityKaifengChina
  3. 3.Institute of Plant Physiology and EcologySIBS, CASShanghaiChina

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