Physiology and Molecular Biology of Plants

, Volume 25, Issue 1, pp 113–121 | Cite as

Analysis of bHLH coding genes of Cicer arietinum during heavy metal stress using biological network

  • Birendra Singh Yadav
  • Ashutosh ManiEmail author
Research Article


bHLH family of transcription factors play important role in regulating many cellular and physiological functions in plants. These proteins are also known to be involved in response to several abiotic stress types. Cicer arietinum is an important source of protein in food across the globe. Considerable differential expression in the bHLH family of proteins during heavy metal exposure in Cicer arietinum was observed by microarray data analysis. The study aimed to construct a Pearson coefficient correlation based network of bHLH coding genes in the plant. Microarray data of Cicer arietinum recorded under cadmium and chromium stress (GSE86807) from GEO at NCBI was used for analysis. The network constructed from expression data set of the 85 bHLH coding genes revealed 10 hub genes that are connected with topological genes. These hub genes are stress responsive genes that may also be regarded as the marker genes for heavy metal response. Our analysis reported a new set of reference genes (hub genes) that have potentially significant role in development of stress tolerant crops.


Biological network Microarray Hub genes Abiotic stress 



BSY is thankful to DST INSPIRE SRF fellowship (IF Code: IF130834). Project is not supported by any funding agency.

Supplementary material

12298_2018_625_MOESM1_ESM.xlsx (4.5 mb)
Supplementary material 1 (XLSX 4613 kb)


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Copyright information

© Prof. H.S. Srivastava Foundation for Science and Society 2018

Authors and Affiliations

  1. 1.Department of BiotechnologyMotilal Nehru National Institute of TechnologyAllahabadIndia

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