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Physiology and Molecular Biology of Plants

, Volume 25, Issue 2, pp 523–532 | Cite as

Effects of glyphosate on soybean metabolism in strains bred for glyphosate-resistance

  • Wei-yu LiEmail author
  • Ping Lu
  • Hao Xie
  • Gui-quan Li
  • Jing-xuan Wang
  • Dong-yu Guo
  • Xing-yu Liang
Research Article
  • 48 Downloads

Abstract

To produce high quality, glyphosate-resistant soybeans, we crossed Jinda 73 and glyphosate-resistant RR1 (Roundup Ready First Generation) (RR1) resulting in 34 hybrid strains. To determine the effects of glyphosate on soybean metabolism, we grew the two parents upto the seedling stage, and measured chlorophyll, soluble sugar, malondialdehyde (MDA), relative conductivity and proline. Then, we treated the plants with glyphosate and measured the same factors again. Results showed that the chlorophyll content of Jinda 73 and RR1 decreased after spraying glyphosate. Glyphosate increased the level of soluble sugar, MDA, relative conductivity and proline in Jinda 73, but had no significant effect on RR1. We determined glyphosate resistance of the parents and the 34 hybrid, offspring strains by documenting the growth response in the field after treatment with glyphosate. Results showed that 29 hybrid, offspring strains have complete glyphosate resistance. Polymerase chain reaction (PCR) shows that the strains which have complete resistance to glyphosate have imported the CP4 5-enolpyhruvylshikimate-3- phosphate synthase (CP4 EPSPS) gene successfully. We selected three high quality, glyphosate-resistant strains (F7-3, F7-16 and F7-21), which had higher protein and oil levels as compared with Jinda 73.

Keywords

Glyphosate Chlorophyll Soluble sugar MDA Conductivity CP4 EPSPS 

Notes

Acknowledgements

This work was partly supported by Beijing Outstanding Talent Training for Young Backbone Individual Projects (No. 2016000020124G049) and Dabeinong Youth Education Foundation (No. 1014115004/020).

Author’s contributions

Wei-yu Li and Gui-quan Li designed the study and prepared the manuscript. Ping Lu, Hao Xie, Jing-xuan Wang, Dong-yu Guo and Xing-yu Liang conducted the experimental work.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

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Copyright information

© Prof. H.S. Srivastava Foundation for Science and Society 2018

Authors and Affiliations

  • Wei-yu Li
    • 1
    Email author
  • Ping Lu
    • 2
  • Hao Xie
    • 1
  • Gui-quan Li
    • 3
  • Jing-xuan Wang
    • 1
  • Dong-yu Guo
    • 1
  • Xing-yu Liang
    • 1
  1. 1.College of Plant Science and TechnologyBeijing University of AgricultureBeijingChina
  2. 2.Beijing University of AgricultureBeijingChina
  3. 3.College of AgricultureShanxi Agriculture UniversityTaiguChina

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