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Development of an efficient in vitro mutagenesis protocol for genetic improvement of saffron (Crocus sativus L.)

  • Mahpara Kashtwari
  • Aijaz A. Wani
  • Manoj K. Dhar
  • Sabbi Jan
  • Azra N. Kamili
Research Article
  • 15 Downloads

Abstract

Saffron (Crocus sativus L) is a triploid (2n = 3x = 24), sterile geophyte which can only be propagated by means of underground vegetative corms. Since corm multiplication does not induce genome variations, therefore, the entire saffron population is expected to have a similar genetic makeup. Keeping in view the economic importance of the plant and the factors responsible for its low yield, the present investigation has been undertaken to establish an in vitro ethyl methanesulfonate (EMS) mutagenesis protocol followed by characterization of the induced variability in the advanced generations. The present report is limited to standardization of in vitro mutagenesis protocol only. Among the mutagenic treatments tested, concentrations ranging from 0.1 to 0.5% EMS showed a varied survival of explants. Based on various growth parameters, the LD50 was calculated to be 0.3% EMS for 3 h. Among the two types of explants analyzed, the corm explant gave better results for in vitro survival and the growth parameters than callus explant. An average of 57.33 and 92.00 daughter cormlets in all EMS treatments as compared to 47.67 and 57.67 daughter cormlets in control, obtained from callus and corm explants respectively, were transferred to the field. The maximum, average daughter cormlet weight was obtained in control (3.01 g, corm explant) followed by 0.1% EMS (2.8 g, corm explant). In general, the growth parameters showed decreasing trend with an increase in EMS concentration in both the explants. The present study has been a significant achievement in the sense that the first mutagenesis protocol for C. sativus has been standardized.

Keywords

Saffron EMS Mutagenesis Callus Shooting Cormlet 

Notes

Acknowledgements

We are highly thankful to Department of Biotechnology, Ministry of Science and Technology, Govt. of India for financial assistance in the project “Induction of variability for genetic improvement of Kashmir Saffron” vide File No. BT/PR5409/PBD/16/977/2012 dated 16.01.2013. Our sincere thanks is also due to in charge Tissue Culture Laboratory for providing necessary laboratory facility and to all those farmers who provided saffron material during our experimental work.

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Copyright information

© Prof. H.S. Srivastava Foundation for Science and Society 2018

Authors and Affiliations

  1. 1.Cytogenetics and Reproductive Biology Laboratory, Department of BotanyUniversity of KashmirSrinagarIndia
  2. 2.Plant Genomics Laboratory, School of BiotechnologyUniversity of JammuJammuIndia
  3. 3.Department of Environmental Science, Centre of Research for DevelopmentUniversity of KashmirSrinagarIndia

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