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Characterization of backcross introgression lines derived from Oryza nivara accessions for photosynthesis and yield

  • Yadavalli Venkateswara Rao
  • Divya Balakrishnan
  • Krishnam Raju Addanki
  • Sukumar Mesapogu
  • Thuraga Vishnu Kiran
  • Desiraju Subrahmanyam
  • Sarla Neelamraju
  • Sitapathi Rao Voleti
Research Article

Abstract

Improvement of photosynthetic traits is a promising strategy to break the yield potential barrier of major food crops. Leaf photosynthetic traits were evaluated in a set of high yielding Oryza sativa, cv. Swarna × Oryza nivara backcross introgression lines (BILs) along with recurrent parent Swarna, both in wet (Kharif) and dry (Rabi) seasons in normal irrigated field conditions. Net photosynthesis (PN) ranged from 15.37 to 23.25 µmol (CO2) m−2 s−1 in the BILs. Significant difference in PN was observed across the seasons and genotypes. Six BILs showed high photosynthesis compared with recurrent parent in both seasons. Chlorophyll content showed minimum variation across the seasons for any specific BIL but significant variation was observed among BILs. Significant positive association between photosynthetic traits and yield traits was observed, but this association was not consistent across seasons mainly due to contrasting weather parameters in both seasons. BILs 166s and 248s with high and consistent photosynthetic rate exhibited stable high yield levels in both the seasons compared to the recurrent parent Swarna. There is scope to exploit photosynthetic efficiency of wild and weedy rice to identify genes for improvement of photosynthetic rate in cultivars.

Keywords

BILs Oryza nivara Photosynthesis Seasonal variation Yield 

Abbreviations

Chl a

Chlorophyll a content (mg g−1 fresh mass)

Chl b

Chlorophyll b content (mg g−1 fresh mass)

Chl a + b

Total chlorophyll content (mg g−1 fresh mass)

PN

Net photosynthetic rate per unit leaf surface area (µmol CO2 m−2 s−1)

Ci

Internal CO2 concentration (µmol CO2 mol−1)

E

Transpiration rate per unit leaf surface area (mmol H2O m−2 s−1)

gs

Stomatal conductance (mol H2O m−2 s−1)

PN/Ci (CE)

Carboxylation efficiency (µmol CO2 mol−1 air)

PN/E (WUE)

Water use efficiency (µmol CO2 mmol−1 H2O)

PN/gs (iWUE)

Intrinsic water use efficiency (µmol CO2 mol−1 H2O)

SLA

Specific leaf area (cm2 mg−1)

SLM

Specific leaf mass (mg cm−2)

TDM

Total dry matter (g plant−1)

HI

Harvest index (%)

Notes

Acknowledgements

This research was conducted in project (ABR/CI/BT/11) on Mapping Quantitative Trait Loci (QTLs) for yield and related traits using backcross inbred lines (BILs) from Elite × Wild crosses of rice (Oryza sativa L.) as part of ICAR- National Professor Project (F.No: Edn/27/4/NP/2012-HRD) funded by Indian Council of Agricultural Research, New Delhi, India. These lines were developed in Department of Biotechnology (DBT) funded project (BT/AB/FG-2 (Ph-II) 2009), New Delhi, India. The authors are highly grateful to the Director, ICAR- IIRR for providing facilities.

Author contributions

DB and SN conceived and planned the work. YVR, AKR, SM and TVK performed phenotypic and genotypic screening. DB and DS analyzed the data. YVR, DB and DS drafted the manuscript. SRV and SN made revisions and approved the final version of the paper.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

12298_2018_575_MOESM1_ESM.docx (167 kb)
Supplementary material 1 (DOCX 167 kb)

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Copyright information

© Prof. H.S. Srivastava Foundation for Science and Society 2018

Authors and Affiliations

  • Yadavalli Venkateswara Rao
    • 1
  • Divya Balakrishnan
    • 1
  • Krishnam Raju Addanki
    • 1
  • Sukumar Mesapogu
    • 1
  • Thuraga Vishnu Kiran
    • 2
  • Desiraju Subrahmanyam
    • 2
  • Sarla Neelamraju
    • 1
  • Sitapathi Rao Voleti
    • 2
  1. 1.ICAR- National Professor Project, ICAR- Indian Institute of Rice ResearchHyderabadIndia
  2. 2.Plant Physiology Section, Department of Crop PhysiologyICAR- Indian Institute of Rice ResearchHyderabadIndia

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