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Physiology and Molecular Biology of Plants

, Volume 24, Issue 4, pp 711–714 | Cite as

Incidence of leaf spot disease on cotton caused by Curvularia verruculosa and role of its hydrolytic enzymes in pathogenesis

  • Leena P. Shirsath
  • Sandip P. Patil
  • Ulhas K. Patil
Short Communication
  • 74 Downloads

Abstract

The finding described in this study is the first report of leaf spot disease of cotton caused by Curvularia verruculosa surveyed in the state of Maharashtra (India). The isolated phytopathogenic fungal strain was identified using morphological characteristics and molecular identification of ITS gene sequence (MF784436) and D1D2 region of LSU gene (KY978073). The ability of fungal strain to secrete hydrolytic enzymes viz., pectinase, xylanase, protease, cellulase and lipase was detected. The secretion profile of hydrolytic enzymes by C. verruculosa was also examined in planta and in vitro. The secretion of cellulase, xylanase and protease was found to be inducible on cotton-stalk powder containing media; while secretion of pectinase and lipase was constitutive in glucose containing medium. The hydrolytic enzymes secretion during etiological progression of disease was detected on cotton leaves at regular interval of 24 h up to 10 days. A significant correlation (P < 0.05) was observed between hydrolytic enzymes secretion and disease severity index. The increased level of hydrolytic enzymes in infected plant sample indicates their role in disease progression. The newly documented fungal phytopathogen Curvularia verruculosa was deposited at National Fungal Culture Collection of India, Pune with accession number of NFCCI-4119.

Keywords

Curvularia verruculosa Transgenic Bt cotton Leaf spot disease Phytopathogen 

Notes

Acknowledgements

The authors are thankful to Dr. S. K. Singh, National Fungal Culture Collection of India, Agharkar Research Institute (NFCCI-ARI) Pune, India for molecular identification and deposition of fungal strain.

References

  1. AICCIP Annual Report (2005–06) All India Coordinated Cotton Improvement Project, Coimbatore, Tamil NaduGoogle Scholar
  2. AICCIP Annual Report (2013–14) All India Coordinated Cotton Improvement Project, Coimbatore, Tamil NaduGoogle Scholar
  3. Bell AA (1999) Diseases of cotton. In: Wayne Smith C, Tom Cothren J (eds) Cotton: origin, history, technology and production. Wiley, New York, pp 553–559Google Scholar
  4. Bellincampi D, Cervone F, Lionetti V (2014) Plant cell wall dynamics and wall related susceptibility in plant–pathogen interactions. Front Plant Sci 5:1–8CrossRefGoogle Scholar
  5. Gour HN, Dube HC (1975) Production of pectic enzymes by Curvularia lunata causing leaf spot of cotton. In: Proceedings of the Indian National Science Academy—Part B: Biological Science, vol 41, pp 480–485Google Scholar
  6. Have TA, Tenberge KB, Benen J, Tudzynski P, Visser J, Van Kan JAL (2002) The contribution of cell wall degrading enzymes to pathogenesis of fungal plant pathogens. Mycota 11:341–358Google Scholar
  7. Huang J, Zheng L, Hsiang T (2005) First report of leaf spot caused by Curvularia verruculosa on Cynodon sp. in Hubei, China. Plant Pathol 54:253CrossRefGoogle Scholar
  8. Islam NF, Borthakur SK (2012) Screening of mycota associated with Aijung rice seed and their effects on seed germination and seedling vigour. Plant Pathol Quar 2:75–85CrossRefGoogle Scholar
  9. Kapat A, Zimand G, Elad Y (1998) Biosynthesis of pathogenicity hydrolytic enzymes by Botrytis cinerea during infection of bean leaves and in vitro. Mycol Res 102:1017–1024CrossRefGoogle Scholar
  10. Kikot GE, Hours RA, Alconada TM (2009) Contribution of cell wall degrading enzymes to pathogenesis of Fusarium graminearum: a review. J Basic Microbiol 49:231–241CrossRefPubMedGoogle Scholar
  11. Kubicek CP, Starr TL, Glass NL (2014) Plant cell wall–degrading enzymes and their secretion in plant-pathogenic fungi. Annu Rev Phytopathol 52:427–451CrossRefPubMedGoogle Scholar
  12. Kusai NA, Azmi MMZ, Zulkifly S, Yusof MT, Zainudin NAIM (2016) Morphological and molecular characterization of Curvularia and related species associated with leaf spot disease of rice in Peninsular Malaysia. Rend Fis 27:205–214CrossRefGoogle Scholar
  13. Sivanesan A (1987) Graminicolous species of Bipolaris, Curvularia, Drechslera, Exserohilum and their teleomorphs. Mycol Pap 158:1–261Google Scholar
  14. Tandon RN, Bilgrami KS (1962) A new pathogenic species of genus Curvularia. Curr Sci 31:254Google Scholar
  15. Watanabe T (2010) Pictorial atlas of soil and seed fungi: morphologies of cultured fungi and key to species. CRC Press, Boca Raton, pp 234–238CrossRefGoogle Scholar
  16. Webster J, Weber R (2007) Introduction to fungi. Cambridge University Press, New York, pp 472–475CrossRefGoogle Scholar
  17. WWF-India report (2012) Cotton market and sustainability in India. WWF-India-Yes Bank, p 7Google Scholar
  18. Zhao B, Yan J, Zhang S, Liu X, Gao Z (2014) Phylogeny and pathogenicity of Fusarium spp. isolated from greenhouse melon soil in Liaoning Province. Saudi J Biol Sci 21:374–379CrossRefPubMedGoogle Scholar

Copyright information

© Prof. H.S. Srivastava Foundation for Science and Society 2018

Authors and Affiliations

  • Leena P. Shirsath
    • 1
  • Sandip P. Patil
    • 1
  • Ulhas K. Patil
    • 1
  1. 1.Department of Microbiology and BiotechnologyR. C. Patel Arts, Commerce and Science CollegeShirpur, District - DhuleIndia

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