Physiology and Molecular Biology of Plants

, Volume 24, Issue 4, pp 711–714 | Cite as

Incidence of leaf spot disease on cotton caused by Curvularia verruculosa and role of its hydrolytic enzymes in pathogenesis

  • Leena P. Shirsath
  • Sandip P. Patil
  • Ulhas K. PatilEmail author
Short Communication


The finding described in this study is the first report of leaf spot disease of cotton caused by Curvularia verruculosa surveyed in the state of Maharashtra (India). The isolated phytopathogenic fungal strain was identified using morphological characteristics and molecular identification of ITS gene sequence (MF784436) and D1D2 region of LSU gene (KY978073). The ability of fungal strain to secrete hydrolytic enzymes viz., pectinase, xylanase, protease, cellulase and lipase was detected. The secretion profile of hydrolytic enzymes by C. verruculosa was also examined in planta and in vitro. The secretion of cellulase, xylanase and protease was found to be inducible on cotton-stalk powder containing media; while secretion of pectinase and lipase was constitutive in glucose containing medium. The hydrolytic enzymes secretion during etiological progression of disease was detected on cotton leaves at regular interval of 24 h up to 10 days. A significant correlation (P < 0.05) was observed between hydrolytic enzymes secretion and disease severity index. The increased level of hydrolytic enzymes in infected plant sample indicates their role in disease progression. The newly documented fungal phytopathogen Curvularia verruculosa was deposited at National Fungal Culture Collection of India, Pune with accession number of NFCCI-4119.


Curvularia verruculosa Transgenic Bt cotton Leaf spot disease Phytopathogen 



The authors are thankful to Dr. S. K. Singh, National Fungal Culture Collection of India, Agharkar Research Institute (NFCCI-ARI) Pune, India for molecular identification and deposition of fungal strain.


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Copyright information

© Prof. H.S. Srivastava Foundation for Science and Society 2018

Authors and Affiliations

  • Leena P. Shirsath
    • 1
  • Sandip P. Patil
    • 1
  • Ulhas K. Patil
    • 1
    Email author
  1. 1.Department of Microbiology and BiotechnologyR. C. Patel Arts, Commerce and Science CollegeShirpur, District - DhuleIndia

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