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Genetic homogeneity revealed in micropropagated Bauhinia racemosa Lam. using gene targeted markers CBDP and SCoT

  • Udit Sharma
  • Manoj K. Rai
  • N. S. Shekhawat
  • Vinod KatariaEmail author
Short communication
  • 12 Downloads

Abstract

Two gene targeted markers i.e. CAAT box-derived polymorphism (CBDP) and start codon targeted (SCoT) polymorphism were applied to analyze the genetic stability of in vitro propagated plants of Bauhinia racemosa Lam. multiplied by enhanced axillary shoot proliferation of mature tree derived nodal explant. Nine randomly selected micropropagated plants of 1 year age were subjected to molecular analysis. The isolated genomic DNA samples were subjected to PCR amplification with a total of 61 primers (25 CBDP and 36 SCoT) out of which 39 primers (21 CBDP and 18 SCoT) produced scorable amplicons. A total of 97 and 88 clear, distinct and reproducible amplicons were produced by CBDP and SCoT primers, respectively. The monomorphic banding pattern obtained through all the tested primers corroborated the true to type nature of in vitro propagated plants of B. racemosa.

Keywords

Gene targeted Genetic stability Molecular markers Plant regeneration Somaclonal variation 

Notes

Acknowledgements

Udit Sharma gratefully acknowledges University Grants Commission, New Delhi for providing financial assistance [F.17-78/2008 (SA-I)] in the form of Junior and Senior research fellowship.

Compliance with ethical standards

Conflict of interest

Authors declare that they have no conflict of interest.

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Copyright information

© Prof. H.S. Srivastava Foundation for Science and Society 2019

Authors and Affiliations

  • Udit Sharma
    • 1
  • Manoj K. Rai
    • 1
    • 2
  • N. S. Shekhawat
    • 1
  • Vinod Kataria
    • 1
    Email author
  1. 1.Biotechnology Unit, Department of Botany (UGC-Centre of Advanced Study)Jai Narain Vyas UniversityJodhpurIndia
  2. 2.Department of Environmental ScienceIndira Gandhi National Tribal UniversityAmarkantakIndia

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