Physiology and Molecular Biology of Plants

, Volume 25, Issue 2, pp 497–509 | Cite as

Different effects of calcium and penconazole on primary and secondary metabolites of Brassica napus under drought

  • Maryam Rezayian
  • Vahid NiknamEmail author
  • Hassan Ebrahimzadeh
Research Article


The effects of penconazole (PEN) and calcium (Ca2+) on physiological and biochemical parameters were investigated in two canola cultivars (RGS003 and Sarigol) under water stress. Drought increased protein content in RGS003, but PEN, Ca2+ and PEN–Ca2+ treatment induced protein content in Sarigol. PEN, Ca2+ and PEN–Ca2+ treatment enhanced soluble sugar content in RGS003. In contrast to Sarigol, drought and PEN treatment induced total phenol content in RGS003. Flavonoid content increased by drought, but Ca2+ and PEN–Ca2+ treatment decreased it in both cultivars. Ca2+ and PEN–Ca2+ treatment enhanced tocopherol content in both cultivars under drought stress. Drought stress increased Phenylalanine ammonia-lyase (PAL) activity in Sarigol. PEN–Ca2+ treatment increased relative expression of PAL and its activity in RGS003. Fatty acid composition was modified by drought, PEN and Ca2+. Saturated fatty acid (stearic acid) content declined but unsaturated fatty acid (oleic acid) content enhanced in both cultivars under drought. The application of PEN and Ca2+ decreased unsaturated fatty acids (linoleic and linolenic acid) in RGS003 under drought. According to our results, PEN and Ca2+ changed physiological and biochemical parameters and therefore these compounds are suggested for reduction of the negative effects of drought stress in canola.


Drought stress PEN Ca2+ Canola Fatty acid Phenolic compound 



The financial support of this research was provided by College of Science, University of Tehran. We thank Dr. Mehrdad Behmanesh and Dr. Najmeh Ahmadian Chashmi.

Author contribution

Maryam Rezayian has contributed in the major bench experiments. Dr. Vahid Niknam and Dr. Hassan Ebrahimzadeh equally designed the experiments. All authors read and approved the manuscript.


  1. Akkol EK, Goger F, Koşar M, Başer KHC (2008) Phenolic composition and biological activities of Salvia halophila and Salvia virgata from Turkey. Food Chem 108:942–949CrossRefGoogle Scholar
  2. Balasundram N, Sundram K, Samman S (2006) Phenolic compounds in plants and agri-industrial by-products: antioxidant activity, occurrence, and potential uses. Food Chem 99:191–203CrossRefGoogle Scholar
  3. Barker AV, Pilbeam DJ (2007) Handbook of plant nutrition. Taylor and Francis Group, New York, pp 121–144Google Scholar
  4. Batistic O, Kudla J (2012) Analysis of calcium signaling pathways in plants. Biochim Biophys Acta 1820:1283–1293CrossRefGoogle Scholar
  5. Berner M, Krug D, Bihlmaier C, Vente A, Muller R, Bechthold A (2006) Genes and enzymes involved in caffeic acid biosynthesis in actinomycete Saccharothrix espanaensis. J Bacteriol 188:2666–2673CrossRefGoogle Scholar
  6. Boudet AM (2007) Evolution and current status of research in phenolic compounds. Phytochemistry 68:2722–2735CrossRefGoogle Scholar
  7. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254CrossRefGoogle Scholar
  8. Bray EA (1997) plant responses to water deficit. Trends Plant Sci 2:48–54CrossRefGoogle Scholar
  9. Bruce WB, Edmeades GO, Barker TC (2002) Molecular and physiological approaches to maize improvement for drought tolerance. J Exp Bot 53:13–25CrossRefGoogle Scholar
  10. Buchanan B, Cruissem W, Jones R (2000) Molecular biology of plants, sec:8. American Society of Plant Physiology, Rockville, pp 379––400Google Scholar
  11. Carvalho MHC (2008) Drought stress and reactive oxygen species: production, scavenging and signaling. Plants Signal Behav 3:156–165CrossRefGoogle Scholar
  12. Chang C, Yang M, Wen H, Chern J (2002) Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal 10:178–182Google Scholar
  13. Conde E, Cadahia E, Garcia-Vallejo M (1995) HPLC analysis of flavonoids and phenolic acids and aldehydes in Eucalyptus spp. Chromatographia 41:657–660CrossRefGoogle Scholar
  14. Crowe JH, Capenter JF, Crowe LM, Anchordoguy TJ (1990) Are freezing and dehydration similar stress vectors? A comparison of modes of interaction of stabilizing solutes with biomolecules. Cryobiology 27:219–231CrossRefGoogle Scholar
  15. Dixon RA, Paiva NL (1995) Stress induced phenylpropanoid metabolism. Plant Cell 7:1085–1097CrossRefGoogle Scholar
  16. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356CrossRefGoogle Scholar
  17. Dure IIL (1993) Structural motifs in Lea proteins. In: Close TJ (ed) Plant response to cellular dehydration during environmental stress. American Society of Plant Physiologists, Rockville, pp 91–103Google Scholar
  18. Elkahoui S, Smaoui A, Mokhtar Zarrouk M, Ghrir R, Limam F (2004) Salt-induced lipid changes in Catharanthus roseus cultured cell suspensions. Phytochemistry 65:1911–1917CrossRefGoogle Scholar
  19. Fletcher RA, Gill A, Davis TD, Sankhla N (2000) Triazoles as plant growth regulators and stress protectants. Hort Rev 24:55–138Google Scholar
  20. Gill KS, Sharma PC (1993) Mechanism of salt injury at seedling and vegetative growth stage in Cajanus cajan (L). Mill sp. Plant Physiol Biochem 20:49–52Google Scholar
  21. Gopi R, Sujatha BM, Rajan SN, Karikalan L, Panneerselvam R (1999) Effect of triadimefon in the sodium chloride stressed cowpea (Vigna unguiculata) seedlings. Indian J Agric Sci 69:743–745Google Scholar
  22. Hassanpour H, Khavari-Nejad RA, Niknam V, Najafi F, Razavi Kh (2012) Effects of penconazole and water deficit stress on physiological and antioxidative responses in pennyroyal (Mentha pulegium L.). Acta Physiol Plant 34:1537–1549CrossRefGoogle Scholar
  23. Hirt H, Shinozaki K (2004) Plant responses to abiotic stress. Springer, BerlinCrossRefGoogle Scholar
  24. Hu XJ, Yu FY, Liu JB, Wan J (2009) Effect of drought stress on soluble sugar content in needles of pinus massoniana seedling from different provenances. J Nanjing For Univ 33:55–59Google Scholar
  25. Ibrahim MH, Jaafar HZE (2011) Photosynthetic capacity, photochemical efficiency and chlorophyll content of three varieties of Labisia pumila Benth exposed to open field and greenhouse growing conditions. Acta Physiol Planta 33:2179–2185CrossRefGoogle Scholar
  26. Ibrahim MFM, Faisal A, Shehata SA (2016) Calcium chloride alleviates water stress in sunflower plants through modifying some physio-biochemical parameters. Am Eurasian J Agric Environ Sci 16:677–693Google Scholar
  27. Jaleel A, Gopi CR, Panneerselvam R (2007a) Alterations in lipid peroxidation, electrolyte leakage and proline metabolism in Catharanthus roseus under treatment with triadimefon, a systemic fungicide. C R Biol 330:905–912CrossRefGoogle Scholar
  28. Jaleel A, Manivannan CP, Sankar B, Kishorekumar A, Gopi R, Somasundaram R, Panneerselvam R (2007b) Induction of drought stress tolerance by ketoconazole in Catharanthus roseus is mediated by enhanced antioxidant potentials and secondary metabolite accumulation. Colloids Surf B 60:201–206CrossRefGoogle Scholar
  29. Jaleel CA, Manivannan P, Kishorekumar A, Sankar B, Panneerselvam R (2007c) Calcium chloride effects on salinity induced oxidative stress, proline metabolism and indole alkaloid accumulation in Catharanthus roseus. C R Biol 330:674–683CrossRefGoogle Scholar
  30. Jaleel A, Gopi CR, Panneerselvam R (2008) Growth and photosynthetic pigments responses of two varieties of Catharanthus roseus to triadimefon treatment. C R Biol 331:272–277CrossRefGoogle Scholar
  31. Ji ZB, Wang JX, Li JW, Xue S, Zhang ML (2009) Dynamic changes of soluble sugar in the seedling of Robinia pseudoacacia under drought stress and rewatering in different seasons. Acta Bot Boreali Occident Sin 29:1358–1363Google Scholar
  32. Kayden HJ, Chow CK, Bjornson LK (1973) Spectrophotometric method for determination of tocopherol in red blood cells. J Lipid Res 14:533–540Google Scholar
  33. Kleinhenz MD, Palta JP (2002) Root zone calcium modulates the response of potato plants to heat stress. Physiol Plant 115:111–118CrossRefGoogle Scholar
  34. Kliebenstein DJ (2004) Secondary metabolites and plant/environment interactions: a view through Arabidopsis thaliana tinged glasses. Plant Cell Environ 27:675–684CrossRefGoogle Scholar
  35. Lattanzio V, Lattanzio VM, Cardinali A (2006) Role of phenolics in the resistance mechanisms of plants against fungal pathogens and insects. Phytochem Adv Res 661:23–67Google Scholar
  36. Luo ZB, Li K, Jiang X, Polle A (2009) Ectomycorrhizal fungus (Paxillus involutus) and hydrogels affect performance of Populus euphratica exposed to drought stress. Ann For Sci 66:106–116CrossRefGoogle Scholar
  37. Ma Q, Turner DW (2006) Osmotic adjustment segregates with and is positively related to seed yield in F3 lines of crosses between Brassica napus and B. juncea subjected to water deficit. Aust J Exp Agric 46:1621–1627CrossRefGoogle Scholar
  38. Ma R, Zhang M, Li B, Du G, Wang J, Chen J (2005) The effects of exogenous Ca2+ on endogenous polyamine levels and drought-resistant traits of spring wheat grown under arid conditions. J Arid Environ 63:177–190CrossRefGoogle Scholar
  39. Ma QQ, Wang W, Li YH, Li DQ, Zou Q (2006) Alleviation of photoinhibition in drought-stressed wheat (Triticum aestivum) by foliarapplied glycinebetaine. J Plant Physiol 163:165–175CrossRefGoogle Scholar
  40. Ma D, Sun D, Wang C, Li Y, Guo T (2014) Expression of flavonoid biosynthesis genes and accumulation of flavonoid in wheat leaves in response to drought stress. Plant Physiol Biochem 80:60–66CrossRefGoogle Scholar
  41. Munne-Bosch S, Alegre L (2002) The function of tocopherols and tocotrienols in plants. Crit Rev Plant Sci 21:31–57CrossRefGoogle Scholar
  42. Munne-Bosche S, Falk J (2004) New insights into the function of tocopherols in plants. Planta 218:323–326CrossRefGoogle Scholar
  43. Nazemi G, Alhani A (2014) The effects of water deficit stress on seed yield and quantitative traits of Canola cultivars. Int J Farm Allied Sci 3:819–822Google Scholar
  44. Neely WC, Martin JM, Barker SA (1988) Products and relative reaction rates of the oxidation of tocopherols with singlet molecular oxygen. Photochem Photobiol 48:423–428CrossRefGoogle Scholar
  45. Pleines S, Friedt W (1988) Breeding for improved C18-fatty acid composition in rapeseed (Brassica napus L.). Fat Sci Technol 5:167–171Google Scholar
  46. Rezayian M, Niknam V, Ebrahimzadeh H (2018) Improving tolerance against drought in canola by penconazole and calcium. Pesticide Biochem Physiol 149:123–136CrossRefGoogle Scholar
  47. Roberson MJ, Holland JF (2004) Production risk of canola in the semi-arid subtropics of Australia. Aust J Agric Res 55:525–538CrossRefGoogle Scholar
  48. Rodriguez-Ruiz J, Belarbi EL-H, Garcia Sanchez GL, Alonso DL (1998) Rapid simultaneous lipid extraction and transesterification for fatty acid analyses. Biotechnol Tech 12:689–691CrossRefGoogle Scholar
  49. Sawhney Y, Singh DP (2002) Effect of chemical desiccation at the post- anthesis stage on some physiological and biochemical changes in the flag leaf of contrasting wheat genotypes. Field crops Res 77:1–6CrossRefGoogle Scholar
  50. Shao HB, Song WY, Chu LY (2008) Advances of calcium signals involved in plant anti-drought. C R Biol 331:587–596CrossRefGoogle Scholar
  51. Sharma P, Dubey RS (2005) Drought induces oxidative stress and enhances the activities of antioxidant enzymes in growing rice seedlings. Plant Growth Regul 46:209–221CrossRefGoogle Scholar
  52. Sridharan R, Manivannan P, Kishorekumar A, Panneerselvam R (2009) Membrane integrity and riboflavin content of Raphanus sativus L. as affected by triazole growth retardants. Mid East J Sci Res 4:52–56Google Scholar
  53. Stewart GR, Lee JA (1974) The role of proline accumulation in halophytes. Planta 120:279–289CrossRefGoogle Scholar
  54. Tuteja N (2009) Integrated calcium signaling in plants. In: Baluška F, Mancuso S (eds) Signaling and communication in plants. Springer, Berlin, pp 29–49CrossRefGoogle Scholar
  55. Unger PW (1982) Time and frequency of irrigation effects on sunflower production and water use. J Am Soil Sci Soc 46:1072–1076CrossRefGoogle Scholar
  56. Wagner GJ (1979) Content and vacuole/extra vacuole distribution of neutral sugars, free amino acids and anthocyanins in protoplasts. Plant Physiol 64:88–93CrossRefGoogle Scholar
  57. Wahid A, Gelani S, Ashraf M, Foolad MR (2007) Heat tolerance in plants: an overview. Environ Exp Bot 61:199–233CrossRefGoogle Scholar
  58. Weidner S, Karalak M, Karamac M, Kosinska A, Amarowicz R (2009) Phenolic compounds and properties of antioxidants in grapevine roots (Vitis vinifera L.) under drought stress followed by recovery. Acta Soc Bot Pol 78:79–103Google Scholar
  59. Worthington RF, Hammons RO (1977) Variability in fatty acid composition among Arachis genotypes: a potential source of product improvement. J Am Oil Chem Soc 54:105–108CrossRefGoogle Scholar
  60. Xiang J, Chen Z, Wang P, Yu L, Li M (2008) Effect of CaCl2 treatment on the changing of drought related physiological and biochemical indexes of Brassica napus. Front Agric China 2:423–427CrossRefGoogle Scholar
  61. Xu C, Li X, Zhang L (2014a) The effect of calcium chloride on growth, photosynthesis, and antioxidant responses of Zoysia japonica under drought conditions. PLoS ONE 8(7):e68214CrossRefGoogle Scholar
  62. Xu W, Peng H, Yang T, Whitaker B, Huang L, Sun J (2014b) Effect of calcium on strawberry fruit flavonoid pathway gene expression and anthocyanin accumulation. Plant Physiol Biochem 82:289–298CrossRefGoogle Scholar
  63. Yordanova RY, Kolev KG, Stoinova ZhG, Popova LP (2004) Changes in the leaf polypeptide patterns of barley plants exposed to soil flooding. Biol Plant 48:301–304CrossRefGoogle Scholar
  64. Yu B, Gong H, Liu Y (1998) Effects of calcium on lipid composition and function of plasma membrane and tonoplast vesicles isolated from roots of barley seedlings under salt stress. J Plant Nutr 21:1589–1600CrossRefGoogle Scholar
  65. Zhao HJ, Tan JF, Qi CM (2007) Photosynthesis of Rehmannia glutinosa subjected to drought stress is enhanced by choline chloride through alleviating lipid peroxidation and increasing proline accumulation. Plant Growth Regul 51:255–262CrossRefGoogle Scholar
  66. Zhou B, Guo Z (2009) Calcium is involved in the abscisic acid-induced ascorbate peroxidase, superoxide dismutase and chilling resistance in Stylosanthes guianensis. Biol Plant 53:63–68CrossRefGoogle Scholar

Copyright information

© Prof. H.S. Srivastava Foundation for Science and Society 2019

Authors and Affiliations

  • Maryam Rezayian
    • 1
  • Vahid Niknam
    • 1
    Email author
  • Hassan Ebrahimzadeh
    • 1
  1. 1.Department of Plant Biology, and Center of Excellence in Phylogeny of Living Organisms in Iran, School of Biology, College of ScienceUniversity of TehranTehranIran

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