Physiology and Molecular Biology of Plants

, Volume 19, Issue 2, pp 261–268 | Cite as

In vitro selection and characterization of polyethylene glycol (PEG) tolerant callus lines and regeneration of plantlets from the selected callus lines in sugarcane (Saccharum officinarum L.)

  • Srinath Rao
  • Jabeen FTZ
Research Article


A system for in vitro selection of drought tolerant callus lines in sugarcane was developed. High molecular weight PEG was used as selective agent. Selected callus line grew better than non-selected callus when grown on different concentrations of PEG. The activity of antioxidant enzymes like CAT, POX, APX and SOD were high in selected callus than in non-selected callus. Osmolytes like proline and ascorbic acid were at higher levels in selected callus than in non-selected callus, however at higher concentrations (20–30 %) of PEG, levels of proline and ascorbic acid decreased. The frequency of organogenesis and number of plantlets decreased in selected callus than in non-selected callus. The results can be used for in vitro screening and manipulations of sugarcane for improvement of drought tolerance


Antioxidant enzymes Callus PEG Selection Proline Sugarcane 



2,4-dichloro phenoxy acitic acid


6-Benzyl aminopurin


α-Napthalene acitic acid








Ascorbate peroxidase


Superoxide dismutase


Murashige and Skoog


Reactive Oxygen Species



The authors thank head department of Botany Gulbarga University Gulbarga for the facilities provided during the course of the investigation,


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Copyright information

© Prof. H.S. Srivastava Foundation for Science and Society 2013

Authors and Affiliations

  1. 1.Department of BotanyGulbarga UniversityGulbargaIndia

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