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Physiology and Molecular Biology of Plants

, Volume 16, Issue 4, pp 375–378 | Cite as

Development of EST-based new SSR markers in seabuckthorn

  • Ankit Jain
  • Rajesh Ghangal
  • Atul Grover
  • Saurabh Raghuvanshi
  • Prakash C. SharmaEmail author
Short Communication

Abstract

EST-based SSR markers were developed by screening a collection of 1584 clustered ESTs of seabuckthorn (Hippophae rhamnoides). PCR primers were designed for the amplification of 30 microsatellite loci. Two to five allelic bands were displayed by nine primer pairs in H. rhamnoides genotypes and by eleven primer pairs in H. salicifolia genotypes. None of the thirty primer pairs detected polymorphism in H. tibetana genotypes. Considering the high polymorphism detected in the tested genotypes and their direct origin from the genic regions, these EST-SSR markers hold immense promise in seabuckthorn genome analysis, molecular breeding and population genetics.

Keywords

Hippophae rhamnoides Expressed sequence tags Simple sequence repeats (SSRs) Cross-amplification 

Notes

Acknowledgements

This research and AJ was supported by Defense Research and Development Organization (DRDO), India through a sponsored project (Project No. LSRB-146/FS/2008). RG thanks Indian Council of Medical Research (ICMR), India for financial support. Authors wish to thank Defense Institute of High Altitude Research (DIHAR), Leh, India for providing genotypes used in this study.

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Copyright information

© Prof. H.S. Srivastava Foundation for Science and Society 2010

Authors and Affiliations

  • Ankit Jain
    • 1
  • Rajesh Ghangal
    • 1
  • Atul Grover
    • 1
    • 3
  • Saurabh Raghuvanshi
    • 2
  • Prakash C. Sharma
    • 1
    Email author
  1. 1.University School of Biotechnology, Guru Gobind Singh Indraprastha UniversityDelhiIndia
  2. 2.Interdisciplinary Center for Plant Genomics, Department of Plant Molecular BiologyUniversity of DelhiNew DelhiIndia
  3. 3.Defense Institute of Bio-Energy ResearchHaldwaniIndia

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