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Physiology and Molecular Biology of Plants

, Volume 16, Issue 2, pp 207–213 | Cite as

Assessment of genetic fidelity of micropropagated date palm (Phoenix dactylifera L.) plants by RAPD and ISSR markers assay

  • Nitish Kumar
  • Arpan R. Modi
  • Amritpal S. Singh
  • Bhavesh B. Gajera
  • Armi R. Patel
  • Mukesh P. Patel
  • Naraynan Subhash
Short Communication

Abstract

RAPD (Random Amplified Polymorphic DNA) and ISSR (Inter-Simple Sequence Repeats) markers assay were employed to validate the genetic stability of date palm (Phoenix dactylifera L.) plants multiplied through somatic embryogenesis with upto forty two in vitro subcultures. Out of the 160 RAPD and 21 ISSR primers screened, 30 RAPD and 12 ISSR primers produced a total of 347 (246 RAPDs + 101 ISSRs) clear, distinct and reproducible amplicons, which were monomorphic across all micropropagated plants (27) studied. Thus, a total 8592 bands (number of plants analysed x number of amplicons with all the primers) were generated which exhibited homogeneous banding patterns with both RAPD and ISSR markers. These results indicate that the micropropagation protocol developed by us for rapid in vitro multiplication is appropriate and suitable for clonal propagation of date palm and corroborated the fact that somatic embryogenesis can also be used as one of the safest modes for production of true-to-type plants.

Keywords

Date palm genetic stability ISSR molecular marker RAPD 

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Copyright information

© Prof. H.S. Srivastava Foundation for Science and Society 2010

Authors and Affiliations

  • Nitish Kumar
    • 1
  • Arpan R. Modi
    • 1
  • Amritpal S. Singh
    • 1
  • Bhavesh B. Gajera
    • 1
  • Armi R. Patel
    • 1
  • Mukesh P. Patel
    • 1
  • Naraynan Subhash
    • 1
  1. 1.Department of Agricultural BiotechnologyAnand Agricultural UniversityAnandIndia

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