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Indian Journal of Clinical Biochemistry

, Volume 34, Issue 1, pp 3–18 | Cite as

Single Cell Omics of Breast Cancer: An Update on Characterization and Diagnosis

  • Shailendra Dwivedi
  • Purvi Purohit
  • Radhieka Misra
  • Malavika Lingeswaran
  • Jeewan Ram Vishnoi
  • Puneet Pareek
  • Sanjeev Misra
  • Praveen SharmaEmail author
Review Article
  • 58 Downloads

Abstract

Breast cancer is recognized for its different clinical behaviors and patient outcomes, regardless of common histopathological features at diagnosis. The heterogeneity and dynamics of breast cancer undergoing clonal evolution produces cells with distinct degrees of drug resistance and metastatic potential. Presently, single cell analysis have made outstanding advancements, overshadowing the hurdles of heterogeneity linked with vast populations. The speedy progression in sequencing analysis now allow unbiased, high-output and high-resolution elucidation of the heterogeneity from individual cell within a population. Classical therapeutics strategies for individual patients are governed by the presence and absence of expression pattern of the estrogen and progesterone receptors and human epidermal growth factor receptor 2. However, such tactics for clinical classification have fruitfulness in selection of targeted therapies, short-term patient responses but unable to predict the long-term survival. In any phenotypic alterations, like breast cancer disease, molecular signature have proven its implication, as we aware that individual cell’s state is regulated at diverse levels, such as DNA, RNA and protein, by multifaceted interplay of intrinsic biomolecules pathways existing in the organism and extrinsic stimuli such as ambient environment. Thus for complete understanding, complete profiling of single cell requires a synchronous investigations from different levels (multi-omics) to avoid incomplete information produced from single cell. In this article, initially we briefed on novel updates of various methods available to explore omics and then we finally pinpointed on various omics (i.e. genomics, transcriptomics, epigenomics, proteomics and metabolomics) data and few special aspects of circulating tumor cells, disseminated tumor cells and cancer stem cells, so far available from various studies that can be used for better management of breast cancer patients.

Keywords

Single cell omics Genomics Transcriptomics Proteomics Molecular sub-typing of breast cancer 

Notes

Acknowledgements

Funding was provided by Science and Engineering Research Board (Grant No. PDF/2015/000322).

Compliance with Ethical Standards

Conflict of interest

All Authors declare that there is no conflict of interest.

Ethical Approval

This article is review article, so does not contain any studies with animals performed by any of the authors.

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Copyright information

© Association of Clinical Biochemists of India 2019

Authors and Affiliations

  • Shailendra Dwivedi
    • 1
  • Purvi Purohit
    • 1
  • Radhieka Misra
    • 2
  • Malavika Lingeswaran
    • 1
  • Jeewan Ram Vishnoi
    • 3
  • Puneet Pareek
    • 4
  • Sanjeev Misra
    • 3
  • Praveen Sharma
    • 1
    Email author
  1. 1.Department of BiochemistryAll India Institute of Medical SciencesJodhpurIndia
  2. 2.Under-graduate Medical ScholarEra’s Lucknow Medical College and HospitalLucknowIndia
  3. 3.Department of Surgical OncologyAll India Institute of Medical SciencesJodhpurIndia
  4. 4.Department of Radio-TherapyAll India Institute of Medical SciencesJodhpurIndia

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