Polymorphisms of BIRC5 Gene is Associated with Chronic HBV Infection in Iranian Population
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Survivin can affect the progression of infection and is considered as a marker of various malignancies. The aim of the study was to investigate the possible association of gene polymorphisms of survivin (-1547A/G, -644C/T, -625 C/G, -241C/T, -31G/C, -141G/C) and chronic hepatitis B infection in Iranian patients. The genotypes of survivin SNPs were investigated by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) method using 100 chronic HBV infected patients (HBV), 40 spontaneously recovered HBV subjects and 100 healthy controls (C). Serum level of survivin was determined using ELISA method. The -1547G, -625C, -241T and -31C alleles were associated with increased susceptibility to chronic hepatitis B infection (P = 0.001, P < 0.001, P = 0.003 and P < 0.001 respectively). Chronic HBV patients with -625CC, -241TT and -31CC genotypes had higher levels of survvin. Survivin -1547A/G, -625 C/G, -241C/T and -31G/C gene polymorphisms may be associated with chronic HBV susceptibility in Iranian HBV patients.
KeywordsChronic hepatitis B Polymorphisms Survivin
Single nucleotide polymorphisms
Hepatitis B virus
Polymerase chain reaction restriction fragment length polymorphism
Enzyme-linked immunosorbent assay
Inhibitor of apoptosis family of proteins
Baculoviral inhibitor of apoptosis repeat containing 5
Reverse transcription polymerase chain reaction
The authors would like to thank all participants who willingly participated in this study. We appreciate all who helped us in this work, especially in the Blood Transfusion Research Center of Zahedan, Iran. This study was approved by the Institutional Ethics Committee of the Zahedan University of Medical Sciences (IR.ZAUMS.REC.1395.159, Grant Number 7868).
BM, ZH, HM-S conceived and co-designed the study, supervised all the experimental design, analyzed the results, and drafted the manuscript. All authors read, modified and approved the final version of the manuscript. These authors equally contributed to this work.
This project was supported by the vice chancellor of Research and Technology of Zahedan University of Medical Sciences (ZUMS) and Infectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences.
Compliance with Ethical Standards
Conflict of interest
The authors declare that they have no conflict of interest.
All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.
Informed consent was written and signed by all individual participants included in the study.
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