Polymorphisms of BIRC5 Gene is Associated with Chronic HBV Infection in Iranian Population

  • Bita Moudi
  • Zahra HeidariEmail author
  • Hamidreza Mahmoudzadeh-Sagheb
Original Research Article


Survivin can affect the progression of infection and is considered as a marker of various malignancies. The aim of the study was to investigate the possible association of gene polymorphisms of survivin (-1547A/G, -644C/T, -625 C/G, -241C/T, -31G/C, -141G/C) and chronic hepatitis B infection in Iranian patients. The genotypes of survivin SNPs were investigated by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) method using 100 chronic HBV infected patients (HBV), 40 spontaneously recovered HBV subjects and 100 healthy controls (C). Serum level of survivin was determined using ELISA method. The -1547G, -625C, -241T and -31C alleles were associated with increased susceptibility to chronic hepatitis B infection (P = 0.001, P < 0.001, P = 0.003 and P < 0.001 respectively). Chronic HBV patients with -625CC, -241TT and -31CC genotypes had higher levels of survvin. Survivin -1547A/G, -625 C/G, -241C/T and -31G/C gene polymorphisms may be associated with chronic HBV susceptibility in Iranian HBV patients.


Chronic hepatitis B Polymorphisms Survivin 



Single nucleotide polymorphisms


Hepatitis B virus


Spontaneously recovered


Polymerase chain reaction restriction fragment length polymorphism


Enzyme-linked immunosorbent assay


Inhibitor of apoptosis family of proteins


Baculoviral inhibitor of apoptosis repeat containing 5


Reverse transcription polymerase chain reaction



The authors would like to thank all participants who willingly participated in this study. We appreciate all who helped us in this work, especially in the Blood Transfusion Research Center of Zahedan, Iran. This study was approved by the Institutional Ethics Committee of the Zahedan University of Medical Sciences (IR.ZAUMS.REC.1395.159, Grant Number 7868).

Author’s Contribution

BM, ZH, HM-S conceived and co-designed the study, supervised all the experimental design, analyzed the results, and drafted the manuscript. All authors read, modified and approved the final version of the manuscript. These authors equally contributed to this work.


This project was supported by the vice chancellor of Research and Technology of Zahedan University of Medical Sciences (ZUMS) and Infectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical Approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed Consent

Informed consent was written and signed by all individual participants included in the study.


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Copyright information

© Association of Clinical Biochemists of India 2019

Authors and Affiliations

  • Bita Moudi
    • 1
    • 2
  • Zahra Heidari
    • 1
    • 2
    Email author
  • Hamidreza Mahmoudzadeh-Sagheb
    • 1
    • 2
  1. 1.Infectious Diseases and Tropical Medicine Research CenterZahedan University of Medical SciencesZahedanIran
  2. 2.Department of Histology, School of MedicineZahedan University of Medical SciencesZahedanIran

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