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Indian Journal of Clinical Biochemistry

, Volume 33, Issue 3, pp 290–296 | Cite as

Plasma Fatty Acid Composition and Estimated Desaturase Activities Reflect Dietary Patterns in Subjects with Metabolic Syndrome

  • N. Pavithra
  • Priyanka S. Bannikoppa
  • Sheila Uthappa
  • Anura V. Kurpad
  • Indu Mani
Original Article
  • 43 Downloads

Abstract

Changes in plasma fatty acid (FA) composition and desaturase activities are observed in metabolic syndrome (MS). However, whether these changes are a reflection of dietary intakes of fats and FAs is not well established. The current study was aimed at assessing plasma FA composition and desaturase enzyme activities as biomarkers of dietary intakes in subjects with MS. Case control study was done on 41 MS patients and was compared with age matched 45 controls. Dietary intakes, anthropometric and clinical parameters were measured. FA composition was analysed using gas chromatography-flame ionisation detector and desaturase enzyme activities were estimated as ratios of product to precursor FAs. Higher levels of 14:0, 16:0, 16:1, 18:1, D9D-18 activity and lower levels of 18:0 and 18:2 n-6 were seen in MS group when compared to controls (p < 0.05). Strong positive correlations were seen between plasma triglyceride (TG) levels and 14:0, 16:0, 16:1, 18:1, total saturated fatty acid, total monounsaturated fatty acid, and D9D activities, while 18:0, 18:2 n-6 and total polyunsaturated fatty acid were negatively correlated with TG. Positive correlations were seen between plasma 14:0, 18:1 and D9D-18 activity with total energy intake and carbohydrate (CHO) intakes but not with fat intake. Plasma FA profile appears to be a better index of total energy intake and CHO intake than fat intake, suggesting it might be a good reflection of endogenous FA metabolism. Changes in FA composition may therefore serve as an early index of dysregulation of FA metabolism, resulting in increased risk of MS.

Keywords

Plasma fatty acids Delta 9-desaturase Dietary carbohydrates Endogenous fatty acid metabolism Metabolic syndrome 

Abbreviations

MS

Metabolic syndrome

CVD

Cardiovascular disorder

FA

Fatty acid

SFA

Saturated fatty acid

MUFA

Monounsaturated fatty acid

PUFA

Polyunsaturated fatty acid

D9D

Delta D9 desaturase

D6D

Delta D6 desaturase

D5D

Delta D5 desaturase

D4D

Delta D4 desaturase

CHO

Carbohydrate

Notes

Acknowledgement

This study was partly funded by Indian Council of Medical Research (ICMR), Government of India (RFC No. 5/4/5-11/10/NCD-II). We gratefully acknowledge all our study volunteers for their kind cooperation.

Author Contributions

All of the authors listed above have contributed to the work and have read, participated in the writing and agreed upon the submitted version of the manuscript.

Compliance with Ethical Standards

Conflict of interest

The authors have no conflict of interest to disclose.

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Copyright information

© Association of Clinical Biochemists of India 2017

Authors and Affiliations

  • N. Pavithra
    • 1
  • Priyanka S. Bannikoppa
    • 1
  • Sheila Uthappa
    • 2
  • Anura V. Kurpad
    • 1
  • Indu Mani
    • 1
  1. 1.Division of Nutrition, St. John’s Research InstituteSt. John’s Medical CollegeBangaloreIndia
  2. 2.Department of BiochemistrySt. John’s Medical CollegeBangaloreIndia

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