SXP–RAL Family Filarial Protein, rWbL2, Prevents Development of DSS-Induced Acute Ulcerative Colitis
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Helminthic infections lead to the release of various molecules which play an important role in modulation of the host immune system. Such filarial proteins with immunomodulatory potential can be used for therapeutic purpose in inflammatory and immune mediated diseases. In the present study, we have explored the prophylactic effect of filarial SXP–RAL family protein of Wuchereria bancrofti i.e. rWbL2 protein in DSS induced inflammatory ulcerative colitis in a mouse model. Prior treatment of rWbL2, followed by induction of colitis, showed significantly reduced disease severity as indicated by the decreased disease manifestations and improved macroscopic and microscopic inflammation. This preventive effect was found to be associated with increased release of anti-inflammatory cytokine IL-10 and decreased release of proinflammatory cytokines IFN-γ, TNF-α, IL-6 and IL-17 by the splenocytes of treated mice. From this study, it can be envisaged that pretreatment with filarial protein, rWbL2, can prevent the establishment of ulcerative colitis in BALB/c mice. The underlying immunological mechanism may involve the up-regulation of Th2 immune response with down-regulation of Th1 response.
KeywordsHelminths Ulcerative colitis Recombinant WbL2 Dextran sodium sulfate
All authors gratefully acknowledge the financial assistance given by Department of Science and Technology (DST) and Department of Biotechnology (DBT), Ministry of Science & Technology, Government of India. Namdev Togre would like to thank Dr. Babasaheb Ambedkar Research and Training Institute (BARTI), Pune, Government of Maharashtra for fellowship.
Funding was provide by Department of Biotechnology , Ministry of Science and Technology (Grant No. BT/PR4988/INF/22/155/2012, dated 01/06/12)
Compliance with ethical standards
Conflict of interest
The authors declare no conflict of interest.
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