Indian Journal of Clinical Biochemistry

, Volume 31, Issue 1, pp 104–107 | Cite as

Study of Association of Leptin and Insulin Resistance Markers in Patients of PCOS

  • Huma Nasrat
  • Surajeet K. Patra
  • Binita Goswami
  • Anju Jain
  • Chitra Raghunandan
Original Article


Polycystic ovary syndrome (PCOS) is a common endocrinological disorder among women of the reproductive age group with long term sequelae which include diabetes mellitus, hypertension and CAD. The present study was conducted to evaluate the association of leptin—an adipokine playing an important role in carbohydrate metabolism and markers of insulin resistance among women with PCOS. Sixty diagnosed cases of PCOS as per Rotterdam criteria were enrolled in this study after informed written consent. Insulin resistance was estimated using the homeostatic model assessment-insulin resistance (HOMA-IR). HOMA-IR was calculated as the product of the fasting plasma insulin value (mU/ml) and the fasting plasma glucose value (mg/dl), divided by 405 and HOMA β was calculated as 360 × [insulin]/([glucose] − 63) % (glucose in mg/dl). Estimation of serum leptin levels was done by ELISA using leptin ELISA kit from (DRG). A positive correlation of serum leptin levels was observed with markers of insulin resistance. Multiple regression analysis with HOMA-IR as dependent variable demonstrated a statistically significant contribution of fasting insulin levels. This study highlights the role of leptin in alterations in carbohydrate metabolism in patients with PCOS.


Leptin PCOS HOMA-IR Insulin resistance 


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Copyright information

© Association of Clinical Biochemists of India 2015

Authors and Affiliations

  • Huma Nasrat
    • 1
  • Surajeet K. Patra
    • 1
  • Binita Goswami
    • 1
  • Anju Jain
    • 1
  • Chitra Raghunandan
    • 2
  1. 1.Department of BiochemistryLady Hardinge Medical CollegeNew DelhiIndia
  2. 2.Department of Obstetrics & GynecologyLady Hardinge Medical CollegeNew DelhiIndia

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