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Indian Journal of Clinical Biochemistry

, Volume 29, Issue 1, pp 69–73 | Cite as

Effect of Resveratrol and Nicotine on PON1 Gene Expression: In Vitro Study

  • Nidhi Gupta
  • Ramesh Kandimalla
  • Kumari Priyanka
  • Gagandip Singh
  • Kiran Dip Gill
  • Surjit SinghEmail author
Original Article

Abstract

Dietary and lifestyle factors have been shown to have a profound effect on paraoxonase-1 (PON1) activity. Cigarette smoke has been shown to inhibit its mass and activity where as resveratrol has been shown to enhance it. We exposed hepatoma derived cell line (HepG2) to resveratrol and nicotine in varying doses and measured PON1 enzymatic activity and PON1 gene expression. In addition, total protein content of HepG2 cells was also measured. Resveratrol in a dose of 15 μmol/l or above significantly increased the PON1 enzyme activity (p > 0.001) where as nicotine in a dose of 1 μmol/l or higher significantly reduced it (p < 0.05). The resveratrol in this dose also enhanced the PON1 gene expression whereas nicotine decreased it as compared to controls. However, the protein conent of cells was not changed suggesting that they were not cytotoxic in the doses used. Till date the antioxidant vitamins have shown disappointing results against LDL oxidation and cardiovascular protection. However, the effect of resveratrol on PON1 gene expression and activity was significant, suggesting increase in PON1 activity and enhanced gene expression may be its alternative mechanism for offering protection against cardiovascular disease and may be an potential pharmacological agent which can be used for this.

Keywords

Paraoxonase1 PON1 gene expression HepG2 cell line Resveratrol Nicotine 

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Copyright information

© Association of Clinical Biochemists of India 2013

Authors and Affiliations

  • Nidhi Gupta
    • 1
  • Ramesh Kandimalla
    • 1
  • Kumari Priyanka
    • 1
  • Gagandip Singh
    • 2
  • Kiran Dip Gill
    • 1
  • Surjit Singh
    • 3
    Email author
  1. 1.Department of BiochemistryPostgraduate Institute of Medical Education and Research, Nehru HospitalChandigarhIndia
  2. 2.Department of CardiologyPostgraduate Institute of Medical Education and Research, Nehru HospitalChandigarhIndia
  3. 3.Department of Internal MedicinePostgraduate Institute of Medical Education and Research, Nehru HospitalChandigarhIndia

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