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Indian Journal of Clinical Biochemistry

, Volume 25, Issue 4, pp 411–418 | Cite as

Evaluation of Oxidative Stress and Antioxidant Status in Pregnant Anemic Women

  • Amit Kumar Mani Tiwari
  • Abbas Ali MahdiEmail author
  • Fatima Zahra
  • Sudarshna Chandyan
  • Vinod Kumar Srivastava
  • Mahendra P. Singh Negi
Original Article

Abstract

The present study was conducted to investigate the oxidant–antioxidant status in iron deficient pregnant anemic women. One hundred thirty pregnant women with iron deficiency anemia (IDA) were divided into three groups, namely mild (50), moderate (50) and severe (30) anemic along with pregnant healthy women as controls (50). The complete blood count, plasma lipid peroxidation products, enzymatic and non-enzymatic antioxidants were measured according to respective protocols. The levels of complete blood count, iron, ferritin along with antioxidant enzymes namely catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase and reduced glutathione were significantly reduced in all IDA groups. However, the level of oxidized glutathione, lipid peroxides, protein carbonyls, conjugated dienes were found significantly increased in all anemic patients. Antioxidant vitamins, namely C, E and A were also found significantly decreased in IDA patients. On the basis of our results, it may be concluded that IDA tends to increase the pro-oxidant components, which may result in various complications including peroxidation of vital body molecules resulting in increased risk for pregnant women as well as fetus.

Keywords

Iron deficiency anemia Oxidants Antioxidants Pregnancy 

Notes

Acknowledgments

Authors acknowledge with thanks the help and guidance of Prof. R. K. Singh (ex-HOD, Biochemistry, CSM Medical University, Lucknow). Authors would like to acknowledge the financial assistance in the form of senior research fellowship given by Council of Science & Technology, U.P.

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Copyright information

© Association of Clinical Biochemists of India 2010

Authors and Affiliations

  • Amit Kumar Mani Tiwari
    • 1
  • Abbas Ali Mahdi
    • 1
    • 6
    Email author
  • Fatima Zahra
    • 2
  • Sudarshna Chandyan
    • 3
  • Vinod Kumar Srivastava
    • 4
  • Mahendra P. Singh Negi
    • 5
  1. 1.Department of BiochemistryC.S.M. Medical UniversityLucknowIndia
  2. 2.Department of Obstetrics and GynecologyEra’s Lucknow Medical College and HospitalLucknowIndia
  3. 3.Department of BiochemistryBundelkhand UniversityJhansiIndia
  4. 4.Department of Community MedicineC.S.M. Medical UniversityLucknowIndia
  5. 5.Biometry DivisionCentral Drug Research InstituteLucknowIndia
  6. 6.Medical Elementology and Free Radical Biology Lab, Department of BiochemistryKing George’s Medical UniversityLucknowIndia

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