Indian Journal of Clinical Biochemistry

, Volume 25, Issue 1, pp 57–63 | Cite as

Utility of serum LDH isoforms in the assessment of mycobacterium tuberculosis induced pathology in TB patients of Sahariya tribe

  • P. R. Sharma
  • S. Jain
  • R. N. K. Bamezai
  • P. K. Tiwari
Original Article

Abstract

The present study was carried out in the Sahariya tribe of Central India, which reportedly have high prevalence of pulmonary tuberculosis. Total serum LDH and its tissue specific isoforms were estimated in TB patients and matched healthy controls to test the utility of LDH as diagnostic marker for tuberculosis. About 210 sputum positive cases and 328 age and sex matched sputum negative controls were recruited. The spectrophotometeric and densitometric analysis of each LDH isoform was carried out in both cases and controls. The mean values of serum LDH were estimated and compared for each class by t-test. The statistical comparisons were made between sputum negative controls and sputum positive cases by Mann-Whitney’s U test. The spectrophotometric estimation of serum LDH revealed significant (P=0.0016) increase in its level in cases (290 IU/L) as compared to controls (248 IU/L). The densitometric analysis of individual LDH isoforms in cases and controls demonstrated significant elevation in LDH1 (P>0.05), LDH2 (P>0.05) and LDH3 (P<0.005) in sputum positive cases in comparison to sputum negative controls. Our study revealed a positive correlation between serum LDH level and the presence of mycobacteria and their load, suggesting utility of LDH as an important diagnostic marker of tuberculosis induced stress, at least in tribal areas lacking access to modern clinical tests.

Key Words

LDH isoforms Tuberculosis Sahariya tribe Acid Fast Bacilli 

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Copyright information

© Association of Clinical Biochemists of India 2010

Authors and Affiliations

  • P. R. Sharma
    • 1
    • 3
  • S. Jain
    • 2
  • R. N. K. Bamezai
    • 3
  • P. K. Tiwari
    • 1
    • 4
  1. 1.Centre for Genomics, School of Studies in Zoology (CG-SSZ)Jiwaji UniversityGwaliorIndia
  2. 2.District HospitalSheopurIndia
  3. 3.National Centre for Applied Human Genetics (NCAHG), SLSJawaharlal Nehru University (JNU)New DelhiIndia
  4. 4.Molecular & Human Genetics School of Studies in ZoologyJiwaji UniversityGwaliorIndia

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