Utility of serum LDH isoforms in the assessment of mycobacterium tuberculosis induced pathology in TB patients of Sahariya tribe
The present study was carried out in the Sahariya tribe of Central India, which reportedly have high prevalence of pulmonary tuberculosis. Total serum LDH and its tissue specific isoforms were estimated in TB patients and matched healthy controls to test the utility of LDH as diagnostic marker for tuberculosis. About 210 sputum positive cases and 328 age and sex matched sputum negative controls were recruited. The spectrophotometeric and densitometric analysis of each LDH isoform was carried out in both cases and controls. The mean values of serum LDH were estimated and compared for each class by t-test. The statistical comparisons were made between sputum negative controls and sputum positive cases by Mann-Whitney’s U test. The spectrophotometric estimation of serum LDH revealed significant (P=0.0016) increase in its level in cases (290 IU/L) as compared to controls (248 IU/L). The densitometric analysis of individual LDH isoforms in cases and controls demonstrated significant elevation in LDH1 (P>0.05), LDH2 (P>0.05) and LDH3 (P<0.005) in sputum positive cases in comparison to sputum negative controls. Our study revealed a positive correlation between serum LDH level and the presence of mycobacteria and their load, suggesting utility of LDH as an important diagnostic marker of tuberculosis induced stress, at least in tribal areas lacking access to modern clinical tests.
Key WordsLDH isoforms Tuberculosis Sahariya tribe Acid Fast Bacilli
Unable to display preview. Download preview PDF.
- 3.Amader E, Pochen EJ. Serum Lactic dehydrogenase activity and radio active scanning in diagnosis of pulmonary embolism. Ann Intern Med 1966;65:1247.Google Scholar
- 4.Agrawal RK. Observations on pleural effusion with special reference to enzyme study and pleural biopsy. MD Thesis (Medicine), University of Indore, Indore: 1968.Google Scholar
- 5.Zimmermann MG, Henry JB. Serum enzyme estimation as an aid to diagnosis. In: Clinical Diagnosis, Philadelphia: WB Saunders Co, 1969:558.Google Scholar
- 6.Brijidshore, Nagrath SP. Serum and pleural fluid lactic dehydrogenase activity in tuberculous pleural effusion. J Ase Phy Ind 1970;18:15.Google Scholar
- 12.Carré PC, Mortenson RL, King TE, Noble PW, Sable CL, Riches DW. Increased expression of the interleukin-8 gene by alveolar macrophages in idiopathic pulmonary fibrosis. A potential mechanism for the recruitment and activation of neutrophils in lung fibrosis. J Clin Invest 1991;88:1802–1810.CrossRefPubMedGoogle Scholar
- 13.Henderson RF, Muggenburg BA, editors. Bronchoalveolar lavage in animals. In: RP. Baughman, Editor. Bronchoalveolar Lavage, Mosby Year Book, St Louis: 1992:265–287.Google Scholar
- 18.Chakma T, Rao PV, Pall S, Kaushal LS, Datta M, Tiwary RS. Survey of pulmonary tuberculosis in a primitive tribe of Madhya Pradesh. Ind J Tub 1996;43:85.Google Scholar
- 20.Cruickshank R, editor. Medical Microbiology, 12th ed. Edinburgh, London and New York: Churchill Livingstone, Medical division of Longmans Group Ltd., 1975: 11pp.Google Scholar
- 21.Rajpal S, Dhingra VK, Agarwal JK. Sputum grading as predictor of treatment outcome in pulmonary tuberculosis. Ind J Tub 2002;49:139.Google Scholar
- 22.RNTCP at a Glance Central TB Division, Directorate General of Health Services, Ministry of Health and Family Welfare, Nirman Bhawan, New Delhi, India, 2006.Google Scholar