Attenuation of oxidative stress in streptozotocin-induced diabetic rats by Eucalyptus globulus

  • Alireza Nakhaee
  • Mohammad Bokaeian
  • Mohsen Saravani
  • Ali Farhangi
  • Azim Akbarzadeh
Original Article


In traditional medicine, Eucalyptus globulus (eucalyptus) was used for the treatment of diabetes mellitus. Hyperglycemia in diabetes has been associated with increased formation of reactive oxygen species (ROS) and oxidative damage to tissue compounds. The aim of this study was to evaluate the effects of eucalyptus in the diet (20 g/Kg) and drinking water (2.5 g/L) on lipid peroxidation, protein oxidation and antioxidant power in plasma and liver homogenate, as well as glycated-Hb (HbA1C) of blood in streptozotocin-induced diabetic rats for a period of 4 weeks. Diabetes induced in rats by a single intraperitoneal injection of streptozotocin (STZ, 65 mg/Kg). At the end of the treatment period, the level of plasma glucose, plasma and liver malondialdehyde (MDA, the main product of lipid peroxidation), protein carbonyl (PC, one of the protein oxidation products) and HbA1C increased and ferric reducing antioxidant power (FRAP) decreased in diabetic rats compared to normal rats. Eucalyptus administration for 4 weeks caused a significant decrease in the plasma glucose levels, plasma and liver MDA, PC and HbA1C, also a concomitant increase in the levels of FRAP in diabetic treated rats. In conclusion, the present study showed that eucalyptus posses antioxidant activities. Eucalyptus probably restores antioxidant power, due to the improved hyperglycemia in streptozotocin-induced diabetic rats.

Key Words

Diabetes mellitus Eucalyptus globulus Ferric reducing antioxidant power Malondialdehyde Protein Carbonyl Glycated-Hb 


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Copyright information

© Association of Clinical Biochemists of India 2009

Authors and Affiliations

  • Alireza Nakhaee
    • 1
  • Mohammad Bokaeian
    • 2
  • Mohsen Saravani
    • 1
  • Ali Farhangi
    • 3
  • Azim Akbarzadeh
    • 3
    • 4
  1. 1.Department of Biochemistry, Faculty of MedicineZahedan University of Medical SciencesZahedanIran
  2. 2.Department of Laboratory Sciences, Faculty of Paramedical sciencesZahedan University of Medical SciencesZahedanIran
  3. 3.Pilot Biotechnology Department of Pasteur institute of IranTehranIran
  4. 4.Pilot Biotechnology DepartmentPasteur Institute of IranTehranIran

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