Indian Journal of Clinical Biochemistry

, Volume 23, Issue 2, pp 191–194

Seasonal variations in markers of stress and oxidative stress in rats

  • Surekha Bhat
  • Guruprasad Rao
  • K. Dilip Murthy
  • P. Gopalakrishna Bhat
Article

Abstract

High ambient temperature has been reported to increase oxidative stress by increasing lipid peroxidation and decreasing antioxidant defence in transition dairy cows. It is also known to cause an increase in plasma cortisol levels in goats, European hedgehog and human volunteers. High levels of glucocorticoids have been reported to decrease blood glutathione and erythrocyte superoxide dismutase activity in rats. Although institutional animal houses in research laboratories of developed countries maintain animals in air-conditioned rooms at constant temperature, the same is not true of animal houses in the developing countries especially those belonging to smaller institutions and this could affect the results of the experiments being conducted on these animals. The present research study was done to assess the effects of seasonal variations on the status of erythrocyte oxidative damage, antioxidant defence and plasma cortisol levels in adult female Wistar rats. Rats were kept in their home cages and were left in non-air-conditioned procedure rooms in two different seasons, Hot season (March-May) and Cool season (June to September). Erythrocyte Thiobarbituric acid reactive substances and plasma cortisol levels were significantly increased in rats exposed to high ambient temperature and humidity of the hot season as compared to the rats of the cool season. Erythrocyte reduced glutathione levels, erythrocyte glutathione peroxidase and superoxide dismutase activities were significantly decreased in the hot season group of rats. The results of our experiments showed that exposure of adult female Wistar rats to high ambient temperature and humidity of the hot season increases neuroendocrine stress, oxidative stress and decreases antioxidant defence in them.

Key Words

Cortisol Temperature Oxidative stress Season Antioxidants 

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References

  1. 1.
    Cross CE, Halliwell B, Borish, ET, Pryor WA, Ames BN, Saul RL et al. Oxygen radicals and human disease. Ann Intern Med 1987; 107: 526–545.PubMedGoogle Scholar
  2. 2.
    Gutteridge JMC. Lipid peroxidation and antioxidants as biomarkers of tissue damage. Clin Chem 1995; 41(12): 1819–1828.PubMedGoogle Scholar
  3. 3.
    Sies H. Antioxidants in disease mechanisms and therapy. In: Advances in Pharmacology. Vol 38. 1997 San Diego, Academic PressGoogle Scholar
  4. 4.
    Aleksandrovskii IA, Poiurovskii MV, Neznamov GG, Seredeniia SB, Krasova EA. Lipid peroxidation in emotional stress and neurotic Disorders. Zhurnal Nevropatologii I pshikiatrii Imeni SS Korsakova 1988; 88(11): 95–101.Google Scholar
  5. 5.
    Orzechowski A, Ostaszewski P, Brodnicka A, Wiczak M, Jank B, Balasmska K, et al. Excess of glucocorticoids impairs whole-body antioxidant status in young rats-relation to the effect of dexamethasone in soleus muscle and spleen. Horm Metab Res 2000; 32(5):174–180.PubMedCrossRefGoogle Scholar
  6. 6.
    Bernabucci U, Ronchi B, Lacetera N, Nardone A. Markers of oxidative status in plasma and erythrocytes of transition dairy cows during hot season. J Dairy Sci 2002; 85(9):2173–2179.PubMedCrossRefGoogle Scholar
  7. 7.
    Hao WW, Qin SZ, Yu QF, Li MG, Ma GX, Zhao H. Effects of heat and noise environments on lipid peroxidation of erythrocyte membrane in pilots. Space Med Eng 2000; 13(1): 52–54.Google Scholar
  8. 8.
    Alila Johanasson A, Erikson L, Soveri T, Laakso M L. Serum cortisol levels in goats exhibit seasonal but not daily rhythmicity. Chronobiol Int 2003; 20(1): 65–79.CrossRefGoogle Scholar
  9. 9.
    Fowler PA. Seasonal endocrine cycles in the European hedgehog, Erinaceus europaeus, J Reprod Ferti 1988; 84(1): 259–272.Google Scholar
  10. 10.
    Hansen AM, Garde AH, Skovgaard LT, Christensen JM. Seasonal and biological variation of urinary epinephrine, norepinephrine and cortisol in healthy women. Clin Chim Acta 2001; 309(1): 25–35.PubMedCrossRefGoogle Scholar
  11. 11.
    Reinberg A, Lagoguey M, Cesselin F, Touitou Y, Legrand JC, Delassalle A, Anrtressian L, Lagoguey A. Circadian and circannual rhythms in plasma hormones and other variables of five healthy young human males. Acta Endocrinol (Copenh) 1978; 88(3): 417–427.Google Scholar
  12. 12.
    Shido O, Sakurada S, Hashimoto T, Fujikake K, Nagasaka T. Increase in plasma thyroid hormone levels during the previous heat exposure time in heat acclimated rats. Can J Physiol Pharmacol 1993; 71(12): 904–909PubMedGoogle Scholar
  13. 13.
    Miura T, Muraoka S, Fujimoto Y. Temperature dependent lipid peroxidation of rat brain homogenate. Res Commun Mol Pathol Pharmacol 1998; 100(1): 117–128.PubMedGoogle Scholar
  14. 14.
    Jain SK, Mc Vie R, Duett J, Herbest JJ. Erythrocyte membrane lipid peroxidation and glycosylated haemoglobin in diabetes. Diabetes 1989; 38: 1539–1543.PubMedCrossRefGoogle Scholar
  15. 15.
    Drabkin DL. Spectrophotometric studies XIV. Crystallographic and optical properties of the haemoglobins of man in comparison with those of the other species. J Biol Chem 1946; 164: 703.Google Scholar
  16. 16.
    Beutler Ernst, Duron Olga, Kelly Barabara, Mikos. Improved method for the determination of blood glutathione. J Lab Clin Med 1963; 61: 882–888.Google Scholar
  17. 17.
    Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of glutathione peroxidase. J Lab Clin Med 1967; 70: 158–169.PubMedGoogle Scholar
  18. 18.
    Beauchamp C, Fridovich I. Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Anal Biochem 1971; 44: 276–287.PubMedCrossRefGoogle Scholar
  19. 19.
    Gowenlock AH, McMurray JR, McLauchlan DM. Measurement of steroids in body fluids-fluorimetric assays in Varley’s Practical Clinical Biochemistry 6th ed, 1988; pp814Google Scholar
  20. 20.
    Bello Klein A, Morgan-Martins MI, Barp J, Llesuy S, Bello AA, Singal PK. Circannual changes in antioxidants and oxidative stress in the heart and liver in rats. Comp Biochem Physiol C 2000; 126: 203–208.CrossRefGoogle Scholar
  21. 21.
    Lamba VJ, Goswami SV, Sundararaj BI. Circannual and circadian variations in plasma levels of steroids (cortisol, estradiol-17 beta, estrone and testosterone) correlated with the annual gonadal cycle in the catfish, Heteropneustes fossilis (Bloch). Gen Comp Endocrinol 1983; 50(2): 205–225.PubMedCrossRefGoogle Scholar
  22. 22.
    Calamari L, Maianti MG, Amendola F, Lombardi G. On some aspects of the oxidative status and on antioxidants in blood of dairy cows during summer in Proc. 13th Associzione Scientifica Produzioni Animali Congress, Piacenza, Italy. 1999; 449–451.Google Scholar
  23. 23.
    Harmon RJ, Lu M, Trammel DS, Smith BA. Influence of heat stress and calving on antioxidant activity in bovine blood [abstract]. J Dairy Sci 1997; 80(Suppl.1): 264.Google Scholar
  24. 24.
    DiGuiseppi J, Fridovich I. The toxicity of molecular oxygen, Crit Rev Toxicol 1984; 12: 315–342.PubMedCrossRefGoogle Scholar

Copyright information

© Association of Clinical Biochemists of India 2008

Authors and Affiliations

  • Surekha Bhat
    • 1
  • Guruprasad Rao
    • 1
  • K. Dilip Murthy
    • 1
    • 2
  • P. Gopalakrishna Bhat
    • 1
    • 3
  1. 1.Department of BiochemistryMelaka Manipal Medical College (Manipal Campus)ManipalIndia
  2. 2.Department of PhysiologyMelaka Manipal Medical College (Manipal Campus)ManipalIndia
  3. 3.Department of BiochemistryKasturba Medical CollegeManipalIndia

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