Effect of Nitric Oxide Donor on Metabolism of Apheresis Platelets
The aim of this study is to investigate the effects of a nitric oxide (NO) donor S-nitrosoglutathione (GSNO) on the metabolism and improvement of preservation quality in apheresis platelets. A GSNO solution with a certain concentration was added into fresh apheresis platelets, and the parameters associated with platelet morphology, metabolism and function were temporally monitored for 7 days. The results showed that the NO level in GSNO group were remarkably higher than those in the control group during the whole storage stage. No significant morphology or function difference was observed between the control and GSNO groups such as Platelet count, platelet distribution width, mean platelet volume and mitochondrial metabolic activity. But in metabolism there are something differences from morphology data: pCO2, pO2, cHCO3 − were also found to have no clear difference between the control and GSNO groups; the lactic acid content, sugar consumption and Lactate dehydrogenase activity in the GSNO group were lower than that in the control group at some time point; and pH values in the GSNO group were higher than the control group. Our study discovered that the NO donor GSNO can reduce the metabolism and maintain the cellular characteristics of platelets in vitro during the platelet storage period.
KeywordsGSNO Nitric oxide donor Platelet storage lesion
We thank Yan Wang and Huihui Feng for contribution to serve the blood donors and PCs collection. This work was supported by the Grants from the Project of Science and Technology Department of Ningbo (No. 2017C50046) and Project of Health Department of Ningbo (No. 2016A17).
QL and GD conceived and designed the experiments; LY, SY, YH and GD performed the experiments and evaluated the results; QL, QL, DX and KH contributed reagents and materials; GD, LY and SY wrote the manuscript.
Compliance with Ethical Standards
Conflict of interest
None of the authors have any conflict of interest to declare.
- 17.Riss TL, Moravec RA (1992) Comparison of MTT, XTT, and a novel tetrazolium compound MTS for in vitro proliferation and chemosensitivity assays. Mol Biol Cell 3:184–194Google Scholar
- 32.Skrzypchak AM, Lafayette NG, Bartlett RH, Zhou Z, Frost MC, Meyerhoff ME, Reynolds MM, Annich GM (2007) Effect of varying nitric oxide release to prevent platelet consumption and preserve platelet function in an in vivo model of extracorporeal circulation. Perfusion 22(3):193–200CrossRefPubMedGoogle Scholar