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BMX activates Wnt/β-catenin signaling pathway to promote cell proliferation and migration in breast cancer

  • Kai Li
  • Wen-ting Pan
  • Yan-bin Ma
  • Xiao-long Xu
  • Yang Gao
  • Yan-qi He
  • Lei Wei
  • Jing-wei ZhangEmail author
Original Article

Abstract

Background

Breast cancer has become a dangerous killer for the female, which seriously threatened women's life, leading to huge pressures to society. The present study assessed the mechanism underlying the involvement of bone marrow tyrosine kinase on chromosome X (BMX) in breast cancer development.

Methods

The expression of BMX was examined by qPCR and immunohistochemistry. The effect of BMX on cell proliferation and migration was detected by Clone formation assay and Transwell assay. In vitro study, the correlation of BMX with Wnt/β-catenin pathway was explored by western blot and TOP/FOP flash assay.

Results

In the present study, we found that BMX was up-regulated in breast cancer, which was associated with the tumor differentiation and TNM stage. Oncogenic BMX enhanced the ability of breast cancer cell proliferation and migration. Furthermore, BMX could up-regulate the protein expression levels of p-β-catenin (Y142), p-β-catenin(Y654) and inhibit the expression level of p-β-catenin (S33/37), thus activating Wnt/β-catenin pathway in MCF-7 and MDA-MB-231 cells. In addition, we revealed that BMX promoted GSK3β phosphorylation, which suppressed the degradation of β-catenin.

Conclusions

In this study, we identified that BMX-activated Wnt/β-catenin signaling pathway, playing an oncogenic role in breast cancer, suggesting that BMX could become a potential treatment target of breast cancer.

Keywords

BMX Breast cancer Wnt/β-catenin pathway Cell proliferation Migration 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (81472765) and the Key Project of Science and Technology Department of Hubei Province (2015CFA070).

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.

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Copyright information

© The Japanese Breast Cancer Society 2019

Authors and Affiliations

  • Kai Li
    • 2
  • Wen-ting Pan
    • 2
  • Yan-bin Ma
    • 2
  • Xiao-long Xu
    • 2
  • Yang Gao
    • 2
  • Yan-qi He
    • 2
  • Lei Wei
    • 2
  • Jing-wei Zhang
    • 1
    Email author
  1. 1.Department of Breast and Thyroid Surgery, Zhongnan Hospital, Hubei Key Laboratory of Tumor Biological Behaviors, Hubei Cancer Clinical Study CenterWuhan UniversityWuhanChina
  2. 2.Department of Pathology and Pathophysiology, Hubei Provincial Key Laboratory of Developmentally Originated Disease, School of Basic Medical SciencesWuhan UniversityWuhanChina

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