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p110α and p110β isoforms of PI3K are involved in protection against H2O2 induced oxidative stress in cancer cells

  • Paramjeet Singh
  • Nasima Bano
  • Md Mehedi Hossain
  • Rafia Basit
  • Mohd Jamal Dar
Original Article
  • 61 Downloads

Abstract

Purpose

Phosphatidylinositol-3 kinases (PI3Ks) are involved in regulating cell growth, proliferation, differentiation, apoptosis and survival. p110α and p110β, two ubiquitously expressed isoforms of PI3K signalling, are involved in growth factor mediated signaling and survival by generating second messengers. Earlier, we have generated GFP-fusion proteins of p110α and p110β and expressed them in normal and cancer cell-lines to investigate their subcellular localization and their role in various activities. Here, we sought to examine the role of p110α and p110β isoforms in protecting MCF-7 breast cancer cells against oxidative stress.

Material methods

We performed cytotoxicity assays, DNA transfection, Plasmid DNA preparation, western blotting, flourscence microscopy and statistical analysis.

Results

To know whether p110α and p110β are involved in protecting MCF-7 breast cancer cells against oxidative stress, we subjected MCF-7 cells to H2O2 treatment and observed a dose dependent decrease in cell viability and a marked increase in the levels of pro-apoptotic markers which include PARP, Bcl-2, Bax and procaspase-9. We then over-expressed recombinant GFP-fusion p110α and p110β proteins in MCF-7 cells and observed a significant decrease in apoptosis and a concomitant increase in pAkt levels.

Conclusion

We report the involvement of p110α and p110β isoforms of Class 1A PI3K signalling in rescue from oxidative stress-induced apoptosis in MCF-7 cells in Akt dependent manner.

Keywords

PI3K signalling Akt Apoptosis Breast cancer 

Notes

Acknowledgements

This work was supported by a grant from the Council of Scientific and Industrial Research (CSIR), Government of India, New Delhi, under HCP0008. This manuscript represents CSIR-IIIM communication number (IIIM/2255/2018).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

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Copyright information

© The Japanese Breast Cancer Society 2018

Authors and Affiliations

  • Paramjeet Singh
    • 1
    • 2
  • Nasima Bano
    • 1
    • 2
  • Md Mehedi Hossain
    • 1
    • 2
  • Rafia Basit
    • 1
    • 2
  • Mohd Jamal Dar
    • 1
    • 2
  1. 1.Academy of Scientific and Innovative ResearchNew DelhiIndia
  2. 2.Cancer Pharmacology DivisionCSIR-Indian Institute of Integrative MedicineJammuIndia

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