Breast Cancer

, Volume 26, Issue 1, pp 93–105 | Cite as

Quantitative proteome and lysine succinylome analyses provide insights into metabolic regulation in breast cancer

  • Chenchen Liu
  • Ying Liu
  • Lei Chen
  • Mingjun Zhang
  • Wei Li
  • Huaidong Cheng
  • Bo ZhangEmail author
Original Article



Breast cancer, the most common invasive cancer and cause of cancer-related death in women worldwide, is a multifactorial, complex disease, and many molecular players and mechanisms underlying the complexity of its clinical behavior remain unknown.


To explore the molecular features of breast cancer, quantitative proteome and succinylome analyses in breast cancer were extensively studied using quantitative proteomics techniques, anti-succinyl lysine antibody-based affinity enrichment, and high-resolution LC–MS/MS analysis.


Our study is the first to detect the regulation of lysine succinylation in breast cancer progression. We identified a novel mechanism by which the pentose phosphate pathway and the endoplasmic reticulum protein processing pathway might be regulated via lysine succinylation in their core enzymes.


These results expand our understanding of tumorigenesis mechanisms and provide a basis for further characterization of the pathophysiological roles in breast cancer progression, laying a foundation for innovative and novel breast cancer drugs and therapies.


Breast cancer Proteomics Lysine succinylation Quantitative analysis Bioinformatics analysis 



We thank the individuals and families who participated in this project. We would also like to thank the participants who helped to collect samples from Hospital No. 1, Anhui Medical University, China. Additionally, we thank the State Key Laboratory Incubation Base of Dermatology, Ministry of National Science and Technology (Hefei, China).

Compliance with ethical standards

Conflict of interest

The authors have declared that no competing interest exists.

Supplementary material

12282_2018_893_MOESM1_ESM.docx (14 kb)
Supplementary material 1 (DOCX 14 KB)
12282_2018_893_MOESM2_ESM.xls (28 kb)
Basic clinical information of three patients (XLS 28 KB)
12282_2018_893_MOESM3_ESM.xlsx (23 kb)
Detailed information on differentially quantified proteins with a threshold fold-change > 1.5 (P <0.05) in human breast cancer (XLSX 23 KB)
12282_2018_893_MOESM4_ESM.xlsx (1 mb)
Identified and quantified proteins in three patients (XLSX 1036 KB)
12282_2018_893_MOESM5_ESM.xlsx (12 kb)
The GO terms of the level 2 distribution of the differentially expressed proteins (XLSX 11 KB)
12282_2018_893_MOESM6_ESM.xlsx (12 kb)
Detailed information on GO-based enrichment of differentially expressed proteins (XLSX 11 KB)
12282_2018_893_MOESM7_ESM.xlsx (23 kb)
Detailed information on GO enrichment-based clustering of differentially expressed proteins (XLSX 22 KB)
12282_2018_893_MOESM8_ESM.xlsx (46 kb)
Detailed information on identified and quantified lysine succinylation sites and proteins (XLSX 45 KB)


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Copyright information

© The Japanese Breast Cancer Society 2018

Authors and Affiliations

  • Chenchen Liu
    • 1
    • 2
  • Ying Liu
    • 1
    • 2
  • Lei Chen
    • 2
  • Mingjun Zhang
    • 2
  • Wei Li
    • 2
  • Huaidong Cheng
    • 2
  • Bo Zhang
    • 1
    • 2
    Email author
  1. 1.School of Life SciencesAnhui Medical UniversityHefeiChina
  2. 2.Department of Oncology, No. 2 HospitalAnhui Medical UniversityHefeiChina

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