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CagL polymorphisms between East Asian and Western Helicobacter pylori are associated with different abilities to induce IL-8 secretion

  • Microbial Pathogenesis and Host-Microbe Interaction
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Abstract

Helicobacter pylori colonizes human gastric mucosa. Its infection is associated with gastric diseases including gastric cancer. CagA is one of the most important toxins produced by H. pylori. It is related to gastric cancer which can be injected into host cells via a type IV secretion system (T4SS). CagL is a structural component of T4SS apparatus, which triggers host cell signaling pathway. It has been reported that CagL polymorphisms may influence the severity of disease development. To explore the contribution of CagL polymorphisms between East Asian and Western H. pylori in pathogenesis, cagL gene in G27 H. pylori was swapped by K74 cagL which is identical to East Asian CagL consensus sequence and by Western 26695 H. pylori, resulting in G27 ΔcagL/cagLK74 and G27 ΔcagL/cagL26695, respectively. Intriguingly, G27 ΔcagL/cagLK74 showed significantly less ability of IL-8 induction than G27 ΔcagL/cagL26695 while displayed similar abilities of CagA phosphorylation, and cell elongation. Taken together, this study suggests that the CagL polymorphism may influence IL-8 induction, and K74 CagL has less ability to induce IL-8 secretion than G27 or 26695 CagL. Further research should address how the different capabilities of IL-8 induction between intraspecies-CagL are associated with the large differences of the incidence of gastric cancer between East Asian and Western countries.

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Acknowledgements

This work was supported by the Korean College of Helicobacter and Upper Gastrointestinal Research Foundation Grant KCHUGR-202001005.

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Author notes

  1. Yun Hui Choi

    Present address: Present address: Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon, 21999, Republic of Korea

  2. Jinmoon Kim

    Present address: Present address: NKMax America, Inc., 3001 Dalmer St.Santa Ana, California, 92705, USA

  3. These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Oral Biology, Oral Science Research Center, Department of Applied Life Science, the Graduate School, BK21 FOUR Project, Yonsei University College of Dentistry, Seoul, 03722, Republic of Korea

    Yun Hui Choi, Jing Lai, Myeong-A Kim, Aeryun Kim, Jinmoon Kim & Jeong-Heon Cha

  2. Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangzhou, Guangdong, 510182, P. R. China

    Hanfu Su, Linhu Ge & Jeong-Heon Cha

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  1. Yun Hui Choi
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Correspondence to Hanfu Su, Linhu Ge or Jeong-Heon Cha.

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Choi, Y.H., Lai, J., Kim, MA. et al. CagL polymorphisms between East Asian and Western Helicobacter pylori are associated with different abilities to induce IL-8 secretion. J Microbiol. 59, 763–770 (2021). https://doi.org/10.1007/s12275-021-1136-2

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