Journal of Microbiology

, Volume 56, Issue 10, pp 693–705 | Cite as

Progress of analytical tools and techniques for human gut microbiome research

  • Eun-Ji Song
  • Eun-Sook Lee
  • Young-Do Nam


Massive DNA sequencing studies have expanded our insights and understanding of the ecological and functional characteristics of the gut microbiome. Advanced sequencing technologies allow us to understand the close association of the gut microbiome with human health and critical illnesses. In the future, analyses of the gut microbiome will provide key information associating with human individual health, which will help provide personalized health care for diseases. Numerous molecular biological analysis tools have been rapidly developed and employed for the gut microbiome researches; however, methodological differences among researchers lead to inconsistent data, limiting extensive share of data. It is therefore very essential to standardize the current methodologies and establish appropriate pipelines for human gut microbiome research. Herein, we review the methods and procedures currently available for studying the human gut microbiome, including fecal sample collection, metagenomic DNA extraction, massive DNA sequencing, and data analyses with bioinformatics. We believe that this review will contribute to the progress of gut microbiome research in the clinical and practical aspects of human health.


gut microbiota microbiome NGS bioinformatics analytical process 


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© The Microbiological Society of Korea and Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Research Group of HealthcareKorea Food Research InstituteWanjuRepublic of Korea
  2. 2.Department of Food BiotechnologyKorea University of Science and TechnologyDaejeonRepublic of Korea

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