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Journal of Microbiology

, Volume 56, Issue 9, pp 683–689 | Cite as

Antiviral activity of Schizonepeta tenuifolia Briquet against noroviruses via induction of antiviral interferons

  • Yee Ching Ng
  • Ye Won Kim
  • Jeong-Su Lee
  • Sung Joon Lee
  • Moon Jung SongEmail author
Virology

Abstract

Human noroviruses are the causative agents of non-bacterial gastroenteritis worldwide. The rapid onset and resolution of disease symptoms suggest that innate immune responses are critical for controlling norovirus infection; however, no effective antivirals are yet available. The present study was conducted to examine the antiviral activities of Schizonepeta tenuifolia Briquet extract (STE) against noroviruses. Treatment of human norovirus replicon-bearing HG23 cells with STE at 5 and 10 mg/ml concentrations resulted in the reduction in the viral RNA levels by 77.2% and 85.9%, respectively. STE had no cytotoxic effects on HG23 cells. Treatment of RAW 264.7 cells infected with murine norovirus 1 (MNV-1), a surrogate virus of human noroviruses, with STE at 10 and 20 µg/ml concentrations resulted in the reduction of viral replication by 58.5% and 84.9%, respectively. STE treatment induced the expression of mRNAs for type I and type II interferons in HG23 cells and upregulated the transcription of interferon-β in infected RAW 264.7 cells via increased phosphorylation of interferon regulatory factor 3, a critical transcription regulator for type I interferon production. These results suggest that STE inhibits norovirus replication through the induction of antiviral interferon production during virus replication and may serve as a candidate antiviral substance for treatment against noroviruses.

Keywords

antiviral activity norovirus Schizonepeta tenuifolia Briquet (ST) natural phytochemicals interferons 

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Copyright information

© The Microbiological Society of Korea and Springer Nature B.V. 2018

Authors and Affiliations

  • Yee Ching Ng
    • 1
  • Ye Won Kim
    • 2
  • Jeong-Su Lee
    • 3
  • Sung Joon Lee
    • 2
  • Moon Jung Song
    • 1
    Email author
  1. 1.Virus-Host Interactions Laboratory, Department of Biosystems and Biotechnology, Division of Biotechnology, College of Life Sciences and BiotechnologyKorea UniversitySeoulRepublic of Korea
  2. 2.Department of Biotechnology, College of Life Sciences and BiotechnologyKorea UniversitySeoulRepublic of Korea
  3. 3.Food Microbiology Division, Food Safety Evaluation DepartmentNational Institute of Food and Drug Safety EvaluationOsongRepublic of Korea

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