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Journal of Microbiology

, Volume 56, Issue 5, pp 324–330 | Cite as

Tardibacter chloracetimidivorans gen. nov., sp. nov., a novel member of the family Sphingomonadaceae isolated from an agricultural soil from Jeju Island in Republic of Korea

  • Hyosun Lee
  • Dong-Uk Kim
  • Sooyeon Park
  • Jung-Hoon Yoon
  • Jae-Hyung Ahn
  • Jong-Ok KaEmail author
Microbial Systematics and Evolutionary Microbiology
  • 125 Downloads

Abstract

A pale yellow bacterial strain, designated JJ-A5T, was isolated form an agricultural soil from Jeju Island in Republic of Korea. Cells of the strain were Gram-stain-negative, motile, flagellated and rod-shaped. The strain grew at 15–30°C, pH 6.0–9.0, and in the presence of 0–1.5% (w/v) NaCl. Growth occurred on R2A, but not on Luria-Bertani agar, nutrient agar, trypticase soy agar and MacConkey agar. The strain utilized alachlor as a sole carbon source for growth. The strain JJ-A5T showed 16S rRNA gene sequence similarities lower than 95.4% with members of the family Sphingomonadaceae. Phylogenetic analysis showed that the strain belongs to the family Sphingomonadaceae and strain JJ-A5T was distinctly separated from established genera of this family. The strain contained Q-10 as dominant ubiquinone and spermidine as major polyamine. The predominant cellular fatty acids were summed feature 8 (C18:1ω7c and/or C18:1ω6c), summed feature 3 (C16:1ω7c and/or C16:1ω6c), 11-methyl C18:1ω7c, C16:0 and C14:0 2-OH. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, sphingoglycolipid, and phosphatidylcholine. The DNA G + C content of the strain was 62.7 mol%. On the basis of the phenotypic, genomic and chemotaxonomic characteristics, strain JJ-A5T is considered to represent a novel genus and species within the family Sphingomonadaceae, for which the name Tardibacter chloracetimidivorans gen. nov., sp. nov. is proposed. The type strain of Tardibacter chloracetimidivorans is JJ-A5T (= KACC 19450T = NBRC 113160T).

Keywords

Tardibacter Tardibacter chloracetimidivorans novel genus novel species polyphasic taxonomy 

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Copyright information

© The Microbiological Society of Korea and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Hyosun Lee
    • 1
  • Dong-Uk Kim
    • 1
  • Sooyeon Park
    • 2
  • Jung-Hoon Yoon
    • 2
  • Jae-Hyung Ahn
    • 3
  • Jong-Ok Ka
    • 1
    Email author
  1. 1.Department of Agricultural Biotechnology and Research Institute of Agriculture and Life SciencesSeoul National UniversitySeoulRepublic of Korea
  2. 2.Department of Food Science and BiotechnologySungkyunkwan UniversitySuwonRepublic of Korea
  3. 3.Agricultural Microbiology Division, National Institute of Agricultural SciencesRural Development AdministrationWanjuRepublic of Korea

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