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Journal of Microbiology

, Volume 55, Issue 10, pp 800–808 | Cite as

Mutation of the cyclic di-GMP phosphodiesterase gene in Burkholderia lata SK875 attenuates virulence and enhances biofilm formation

  • Hae-In Jung
  • Yun-Jung Kim
  • Yun-Jung Lee
  • Hee-Soo Lee
  • Jung-Kee Lee
  • Soo-Ki KimEmail author
Microbial Genetics, Genomics and Molecular Biology

Abstract

Burkholderia sp. is a gram-negative bacterium that commonly exists in the environment, and can cause diseases in plants, animals, and humans. Here, a transposon mutant library of a Burkholderia lata isolate from a pig with swine respiratory disease in Korea was screened for strains showing attenuated virulence in Caenorhabditis elegans. One such mutant was obtained, and the Tn5 insertion junction was mapped to rpfR, a gene encoding a cyclic di-GMP phosphodiesterase that functions as a receptor. Mutation of rpfR caused a reduction in growth on CPG agar and swimming motility as well as a rough colony morphology on Congo red agar. TLC analysis showed reduced AHL secretion, which was in agreement with the results from plate-based and bioluminescence assays. The mutant strain produced significantly more biofilm detected by crystal violet staining than the parent strain. SEM of the mutant strain clearly showed that the overproduced biofilm contained a filamentous structure. These results suggest that the cyclic di-GMP phosphodiesterase RpfR plays an important role in quorum sensing modulation of the bacterial virulence and biofilm formation.

Keywords

quorum sensing Burkholderia lata transposon Caenorhabditis elegans c-di-GMP phosphodiesterase BDSF 

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Copyright information

© The Microbiological Society of Korea and Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Hae-In Jung
    • 1
  • Yun-Jung Kim
    • 1
  • Yun-Jung Lee
    • 1
  • Hee-Soo Lee
    • 2
  • Jung-Kee Lee
    • 3
  • Soo-Ki Kim
    • 1
    Email author
  1. 1.Department of Animal Science and TechnologyKonkuk UniversitySeoulRepublic of Korea
  2. 2.National Veterinary Research and Quarantine ServiceAnyangRepublic of Korea
  3. 3.Department of Life Science and Genetic EngineeringPaichai UniversityDaejeonRepublic of Korea

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