Journal of Microbiology

, Volume 55, Issue 9, pp 678–683 | Cite as

Spirosoma daeguensis sp. nov., isolated from beach soil

  • Nabil Elderiny
  • Leonid N. Ten
  • Jae-Jin Lee
  • Seung-Yeol Lee
  • Sangkyu Park
  • Young-Je Cho
  • Myung Kyum Kim
  • Hee-Young Jung
Article
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Abstract

A Gram-stain-negative, non-motile, non-spore-forming, rod-shaped, aerobic bacterium, designated 15J9-6T, was isolated from beach soil on Jeju Island, South Korea. Strain 15J9-6T, grew at 10–30°C (optimum growth at 25°C) and pH 7–8 (optimum growth at pH 7) on R2A, NA, and TSA agar. Phylogenetically, the strain was closely related to members of the genus Spirosoma (92.3–90.1% 16S rRNA gene sequence similarities) and showed highest sequence similarity to Spirosoma panaciterrae DSM 21099T (92.3%). The G+C content of the genomic DNA of strain 15J9-6T was 45.7 mol%. The strain contained phosphatidylethanolamine, two unidentified aminophospholipids, an unidentified phospholipid, and an unidentified lipid as the major polar lipids; menaquinone MK-7 as the predominant respiratory quinone and summed feature 3 (C16:1 ω6c/C16:1 ω7c; 30.1%), C16:1 ω5c (23.1%), iso C15:0 (13.3%), and C16:0 (8.4%) as the major fatty acids which supported the affiliation of strain 15J9-6T to the genus Spirosoma. The results of physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 15J9-6T from recognized Spirosoma species. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain 15J9-6T represents a novel species of the genus Spirosoma, for which the name Spirosoma daeguensis sp. nov. is proposed. The type strain is 15J9-6T (=KCTC 52036T =JCM 31995T)

Keywords

Spirosoma Bacteroidetes polyphasic taxonomy 
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Copyright information

© The Microbiological Society of Korea and Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Nabil Elderiny
    • 1
  • Leonid N. Ten
    • 1
  • Jae-Jin Lee
    • 1
  • Seung-Yeol Lee
    • 1
  • Sangkyu Park
    • 1
  • Young-Je Cho
    • 2
  • Myung Kyum Kim
    • 3
  • Hee-Young Jung
    • 1
    • 4
  1. 1.School of Applied BiosciencesKyungpook National UniversityDaeguRepublic of Korea
  2. 2.School of Food Science and Biotechnology/Food and Bio-Industry Research InstituteKyungpook National UniversityDaeguRepublic of Korea
  3. 3.Department of Bio and Environmental TechnologySeoul Women’s UniversitySeoulRepublic of Korea
  4. 4.Institute of Plant MedicineKyungpook National UniversityDaeguRepublic of Korea

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