Journal of Microbiology

, Volume 55, Issue 8, pp 640–647 | Cite as

Effect of amikacin on cell wall glycopeptidolipid synthesis in Mycobacterium abscessus

  • So-Young Lee
  • Hee-Youn Kim
  • Byoung-Jun Kim
  • Hong Kim
  • Seung-hyeok Seok
  • Bum-Joon Kim
  • Yoon-Hoh Kook
Article
First Online:
Received:
Revised:
Accepted:

Abstract

Cultivation of the smooth colony Mycobacterium abscessus at the sub-minimum inhibitory concentration (MIC) of amikacin changed its growth pattern including its colony morphology (smooth to rough) and cell arrangement (dispersed to cord formation). In addition, reduced sliding motility and biofilm formation were observed. The amount of glycogpetidolipid (GPL) and mRNA expression of key genes involved in GPL synthesis were decreased in the amikacin-treated M. abscessus strain. An in vitro infection assay revealed that the amikacin-treated smooth M. abscessus strain induced more pro-inflammatory cytokines (TNF-α, IL-1β, IL-6) than that of the smooth strain in murine macrophage cells. These results suggest that long-term exposure to a low concentration of amikacin causes a physical change in the cell wall which may increase its virulence.

Keywords

Mycobacterium abscessus glycopeptidolipid (GPL) amikacin biofilm 
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Supplementary material

12275_2017_6503_MOESM1_ESM.pdf (129 kb)
Supplementary data Fig. S1. Comparison of cell wall components and GPLs extracted from M. abscessus

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Copyright information

© The Microbiological Society of Korea and Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • So-Young Lee
    • 1
  • Hee-Youn Kim
    • 1
  • Byoung-Jun Kim
    • 1
  • Hong Kim
    • 1
  • Seung-hyeok Seok
    • 1
  • Bum-Joon Kim
    • 1
    • 2
    • 3
  • Yoon-Hoh Kook
    • 1
  1. 1.Department of Microbiology and ImmunologySeoul National University College of MedicineSeoulRepublic of Korea
  2. 2.Department of Biomedical SciencesSeoul National University College of MedicineSeoulRepublic of Korea
  3. 3.Cancer Research Institute, and Institute of Endemic Diseases, Seoul National University Medical Research Center (SNUMRC)Seoul National University College of MedicineSeoulRepublic of Korea

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