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Journal of Microbiology

, Volume 54, Issue 12, pp 838–845 | Cite as

Soil pH and electrical conductivity are key edaphic factors shaping bacterial communities of greenhouse soils in Korea

  • Jeong Myeong Kim
  • An-Sung Roh
  • Seung-Chul Choi
  • Eun-Jeong Kim
  • Moon-Tae Choi
  • Byung-Koo Ahn
  • Sun-Kuk Kim
  • Young-Han Lee
  • Jae-Ho Joa
  • Seong-Soo Kang
  • Shin Ae Lee
  • Jae-Hyung Ahn
  • Jaekyeong Song
  • Hang-Yeon WeonEmail author
Microbial Ecology and Environmental Microbiology

Abstract

Soil microorganisms play an essential role in soil ecosystem processes such as organic matter decomposition, nutrient cycling, and plant nutrient availability. The land use for greenhouse cultivation has been increasing continuously, which involves an intensive input of agricultural materials to enhance productivity; however, relatively little is known about bacterial communities in greenhouse soils. To assess the effects of environmental factors on the soil bacterial diversity and community composition, a total of 187 greenhouse soil samples collected across Korea were subjected to bacterial 16S rRNA gene pyrosequencing analysis. A total of 11,865 operational taxonomic units at a 97% similarity cutoff level were detected from 847,560 sequences. Among nine soil factors evaluated; pH, electrical conductivity (EC), exchangeable cations (Ca2+, Mg2+, Na+, and K+), available P2O5, organic matter, and NO3-N, soil pH was most strongly correlated with bacterial richness (polynomial regression, pH: R2 = 0.1683, P < 0.001) and diversity (pH: R2 = 0.1765, P < 0.001). Community dissimilarities (Bray-Curtis distance) were positively correlated with Euclidean distance for pH and EC (Mantel test, pH: r = 0.2672, P < 0.001; EC: r = 0.1473, P < 0.001). Among dominant phyla (> 1%), the relative abundances of Proteobacteria, Gemmatimonadetes, Acidobacteria, Bacteroidetes, Chloroflexi, and Planctomycetes were also more strongly correlated with pH and EC values, compared with other soil cation contents, such as Ca2+, Mg2+, Na+, and K+. Our results suggest that, despite the heterogeneity of various environmental variables, the bacterial communities of the intensively cultivated greenhouse soils were particularly influenced by soil pH and EC. These findings therefore shed light on the soil microbial ecology of greenhouse cultivation, which should be helpful for devising effective management strategies to enhance soil microbial diversity and improving crop productivity.

Keywords

greenhouse soil bacterial community pH electrical conductivity 

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Copyright information

© The Microbiological Society of Korea and Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Jeong Myeong Kim
    • 1
  • An-Sung Roh
    • 2
  • Seung-Chul Choi
    • 3
  • Eun-Jeong Kim
    • 4
  • Moon-Tae Choi
    • 5
  • Byung-Koo Ahn
    • 6
  • Sun-Kuk Kim
    • 7
  • Young-Han Lee
    • 8
  • Jae-Ho Joa
    • 9
  • Seong-Soo Kang
    • 10
  • Shin Ae Lee
    • 1
  • Jae-Hyung Ahn
    • 1
  • Jaekyeong Song
    • 1
  • Hang-Yeon Weon
    • 1
    Email author
  1. 1.National Institutes of Agricultural Sciences (NIAS)Rural Development Administration (RDA)WanjuRepublic of Korea
  2. 2.Gyeonggi-do Agricultural Research and Extension Service (ARES)HwaseongRepublic of Korea
  3. 3.Gangwon-do ARESChuncheonRepublic of Korea
  4. 4.Chungcheongbuk-do ARESCheongjuRepublic of Korea
  5. 5.Chungcheongnam-do ARESYesanRepublic of Korea
  6. 6.Jeollabuk-do ARESIksanRepublic of Korea
  7. 7.Jeollanam-do ARESNajuRepublic of Korea
  8. 8.Gyeongsangnam-do ARESJinjuRepublic of Korea
  9. 9.Research Institute of Climate Change and Agriculture, National Institute of Horticultural and Herbal ScienceRDAJejuRepublic of Korea
  10. 10.Soil & Fertilization Division, NIASRDAWanjuRepublic of Korea

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