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Journal of Microbiology

, Volume 52, Issue 6, pp 504–509 | Cite as

Profiling of the bacteria responsible for pyogenic liver abscess by 16S rRNA gene pyrosequencing

  • Yun Gyu Song
  • Sang Gun Shim
  • Kwang Min Kim
  • Dong-Hae Lee
  • Dae-Soo Kim
  • Sang-Haeng Choi
  • Jae-Young Song
  • Hyung-Lyun Kang
  • Seung-Chul Baik
  • Woo-Kon Lee
  • Myung-Je ChoEmail author
  • Kwang-Ho Rhee
Article

Abstract

Pyogenic liver abscess (PLA) is a severe disease with considerable mortality and is often polymicrobial. Understanding the pathogens that cause PLA is the basis for PLA treatment. Here, we profiled the bacterial composition in PLA fluid by pyrosequencing the 16S ribosomal RNA (rRNA) gene based on next-generation sequencing (NGS) technology to identify etiological agents of PLA and to provide information of their 16S rRNA sequences for application to DNA-based techniques in the hospital. Twenty patients with PLA who underwent percutaneous catheter drainage, abscess culture, and blood culture for isolates were included. Genomic DNAs from abscess fluids were subjected to polymerase chain reaction and pyrosequencing of the 16S rRNA gene with a 454 GS Junior System. The abscess and blood cultures were positive in nine (45%) and four (20%) patients, respectively. Pyrosequencing of 16S rRNA gene showed that 90% of the PLA fluid samples contained single or multiple genera of known bacteria such as Klebsiella, Fusobacterium, Streptococcus, Bacteroides, Prevotella, Peptostreptococcus, unassigned Enterobacteriaceae, and Dialister. Klebsiella was predominantly found in the PLA fluid samples. All samples that carried unassigned bacteria had 26.8% reads on average. We demonstrated that the occurrence of PLA was associated with eight known bacterial genera as well as unassigned bacteria and that 16S rRNA gene sequencing was more useful than conventional culture methods for accurate identification of bacterial pathogens from PLA.

Keywords

pyogenic liver abscess abscess culture metagenomics 16S rRNA gene sequencing next generation sequencing 

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Copyright information

© The Microbiological Society of Korea and Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Yun Gyu Song
    • 1
  • Sang Gun Shim
    • 2
  • Kwang Min Kim
    • 2
  • Dong-Hae Lee
    • 5
  • Dae-Soo Kim
    • 3
  • Sang-Haeng Choi
    • 4
  • Jae-Young Song
    • 5
  • Hyung-Lyun Kang
    • 5
  • Seung-Chul Baik
    • 5
  • Woo-Kon Lee
    • 5
  • Myung-Je Cho
    • 5
    Email author
  • Kwang-Ho Rhee
    • 5
  1. 1.Department of RadiologySungkyunkwan University School of MedicineChangwonRepublic of Korea
  2. 2.Department of Internal Medicine, Samsung Changwon HospitalSungkyunkwan University School of MedicineChangwonRepublic of Korea
  3. 3.Korea Research Institute of Bioscience and BiotechnologyHuman Derived Material CenterDaejeonRepublic of Korea
  4. 4.Unitech Science Co., Ltd.DaejeonRepublic of Korea
  5. 5.5Department of MicrobiologyGyeongsang National University School of MedicineJinjuRepublic of Korea

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