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Journal of Microbiology

, Volume 52, Issue 4, pp 340–344 | Cite as

Antiviral effect of flavonol glycosides isolated from the leaf of Zanthoxylum piperitum on influenza virus

  • Song-Yi Ha
  • Hana Youn
  • Chang-Seon Song
  • Se Chan Kang
  • Jong Jin Bae
  • Hee Tae Kim
  • Kwang Min Lee
  • Tae Hoon Eom
  • In Su Kim
  • Jong Hwan KwakEmail author
Virology

Abstract

The ethanol extract of Zanthoxylum piperitum (L.) DC. showed in vitro antiviral activity against influenza A virus. Three flavonol glycosides were isolated from the EtOAc fraction of Z. piperitum leaf by means of activity-guided chromatographic separation. Structures of isolated compounds were identified as quercetin 3-O-β-D-galactopyranoside (1), quercetin 3-O-α-L-rhamnopyranoside (2), kaempferol 3-O-α-L-rhamnopyranoside (3) by comparing their spectral data with literature values. The anti-influenza viral activity of isolates was evaluated using a plaque reduction assay against influenza A/NWS/33 (H1N1) virus. The compounds also were subjected to neuraminidase inhibition assay in influenza A/NWS/33 virus. Compounds 1–3 exhibited antiviral activity against an influenza A virus in vitro, and inhibited the neuraminidase activity at relatively high concentrations.

Keywords

Zanthoxylum piperitum Rutaceae flavonol glycoside anti-influenza activity neuraminidase inhibitor 

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Copyright information

© The Microbiological Society of Korea and Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Song-Yi Ha
    • 1
  • Hana Youn
    • 2
  • Chang-Seon Song
    • 2
  • Se Chan Kang
    • 3
  • Jong Jin Bae
    • 4
  • Hee Tae Kim
    • 1
  • Kwang Min Lee
    • 1
  • Tae Hoon Eom
    • 1
  • In Su Kim
    • 1
  • Jong Hwan Kwak
    • 1
    Email author
  1. 1.School of PharmacySungkyunkwan UniversitySuwonRepublic of Korea
  2. 2.College of Veterinary MedicineKonkuk UniversitySeoulRepublic of Korea
  3. 3.Department of Life ScienceGachon UniversitySungnamRepublic of Korea
  4. 4.Department of Oriental PharmacyWoosuk UniversityJeonjuRepublic of Korea

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