Journal of Microbiology

, Volume 51, Issue 4, pp 490–498

Dysregulation of KSHV replication by extracts from Carthamus tinctorius L.

  • Han Lee
  • Hyosun Cho
  • Myoungki Son
  • Gi-Ho Sung
  • Taeho Lee
  • Sang-Won Lee
  • Yong Woo Jung
  • Yu Su Shin
  • Hyojeung Kang
Microbial Pathogenesis and Host-Microbe Interaction

DOI: 10.1007/s12275-013-3282-7

Cite this article as:
Lee, H., Cho, H., Son, M. et al. J Microbiol. (2013) 51: 490. doi:10.1007/s12275-013-3282-7

Abstract

Carthamus tinctorius L. (CT) is traditionally used to reduce ailments from diseases of the musculoskeletal system and connective tissue and diseases of blood circulation and the cardiovascular system. Flower extracts from CT are known to have antibacterial activity, anti-inflammatory activity, and to inhibit tumor promotion in mouse skin carcinogenesis. In order to discover new antiviral agents from CT extracts, we tested whether CT extracts contain antiviral activity against gammaherpesvirus infection. This study demonstrated that treatment with CT extracts disrupted KSHV latency in the viral-infected host cells, iSLK-BAC16. n-Hexane and EtOH fractions of CT extracts critically affected at least two stages of the KHSV life-cycle by abnormally inducing KSHV lytic reactivation and by severely preventing KSHV virion release from the viral host cells. In addition to the effects on KSHV itself, CT extract treatments induced cellular modifications by dysregulating cell-cycle and producing strong cytotoxicity. This study demonstrated for the first time that CT extracts have antiviral activities that could be applied to development of new anti-gammaherpesviral agents.

Keywords

Carthamus tinctorius L. Kaposi’s sarcoma-associated herpesvirus extracts latency lytic reactivation 

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12275_2013_3282_MOESM1_ESM.pdf (234 kb)
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Copyright information

© The Microbiological Society of Korea and Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Han Lee
    • 1
  • Hyosun Cho
    • 2
  • Myoungki Son
    • 1
  • Gi-Ho Sung
    • 3
  • Taeho Lee
    • 4
  • Sang-Won Lee
    • 5
  • Yong Woo Jung
    • 6
  • Yu Su Shin
    • 5
  • Hyojeung Kang
    • 1
  1. 1.College of Pharmacy and Research Institute of Pharmaceutical Sciences, Institute for MicroorganismsKyungpook National UniversityDaeguRepublic of Korea
  2. 2.College of PharmacyDuksung Women’s UniversitySeoulRepublic of Korea
  3. 3.Mushroom Research Division, National Institute of Horticultural and Herbal ScienceRural Development AdministrationSuwonRepublic of Korea
  4. 4.Research Center, Dong-a Pharm. Co., LTD.YonginRepublic of Korea
  5. 5.Department of Medicinal Crop Research, National Institute of Horticultural and Herbal ScienceRural Development AdministrationEumseongRepublic of Korea
  6. 6.College of PharmacyKorea UniversitySejongRepublic of Korea

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